Hard to say what's going on. Perhaps you could upload your new mascot result file to the files area at http://groups.google.com/group/spctools-discuss?hl=en. and someone can have a look.
Brian On Fri, Jun 18, 2010 at 11:09 AM, Fay Wang <[email protected]> wrote: > Hi all, > > I posted my questions yesterday, but they were a bit too long to read, > so I shortened it (still seems long). > > I am trying to use TPP (4.3.1) to run some cICAT data I collected a > while ago. All these cICAT data have been searched by Mascot (I have > all the .dat files). So far I have not got my data run by TPP on my > computer. Here are some questions. > > 1. If I have 1 mzXML file, a Mascot.dat file, and a database.fasta > file, are they enough to run TPP? (I guess so). > > 2. Does the database.fasta file has to be the exactly same > database.fasta file when I did Mascot searches (my data are a bit > old). I use SwissProt database. As I recall, when you update it, the > newer database overwrite the older one. So, can I use the Mascot.dat > files (searched against a older version SwissProt database) along with > the current database (newer version) to get TPP run? If not, where can > I download those older databases? I can not find them online. > > 3. I redid Mascot search anyway. With this new .dat file and the > SwissProt database.fasta file (for generating this .dat file), the TPP > still did not run. I got following message: > > c:/Inetpub/wwwroot/ISB/data/SSM4-1/F030337.pep.xml [ Unreadable! ] > > command "c:\Inetpub\tpp-bin\Mascot2XML c:/Inetpub/wwwroot/ISB/data/ > SSM4-1/F030337.dat -Dc:/Inetpub/wwwroot/ISB/data/SwissProt_57.12.fasta > -Etrypsin" failed: Operation not permitted > Command FAILED > > I do not know how to fix it. It seems some permission issue? I looked > up a lot of old posts, seems no one ever mentioned this problem. Maybe > it is too simple to ask? > > Any suggestion? Thanks a lot! > > Fay > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To post to this group, send email to [email protected]. > To unsubscribe from this group, send email to > [email protected]<spctools-discuss%[email protected]> > . > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. > > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
