run_in c:/Inetpub/wwwroot/ISB/data/mito/mito_p2_phospho_hetero_II; c: \Inetpub\tpp-bin\xinteract -Ninteract.pep.xml -p0.05 -l7 -O -dR_ c:/ Inetpub/wwwroot/ISB/data/mito/mito_p2_phospho_hetero_II/ mito_p2_phospho_hetero_II_95ug_out.pep.xml --------------------------------------------------------------------------------
c:\Inetpub\tpp-bin\xinteract (TPP v4.4 VUVUZELA (4.4.0 RC4) rev 0, Build 201008031534 (MinGW)) running: "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml" "c:/ Inetpub/wwwroot/ISB/data/mito/mito_p2_phospho_hetero_II/ mito_p2_phospho_hetero_II_95ug_out.pep.xml" -L"7"" file 1: c:/Inetpub/wwwroot/ISB/data/mito/mito_p2_phospho_hetero_II/ mito_p2_phospho_hetero_II_95ug_out.pep.xml processed altogether 8449 results results written to file c:/Inetpub/wwwroot/ISB/data/mito/ mito_p2_phospho_hetero_II/interact.pep.xml direct your browser to http://localhost/ISB/data/mito/mito_p2_phospho_hetero_II/interact.pep.shtml command completed in 2 sec running: "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml" MINPROB=0.05 DECOY=R_" Using Decoy Label "R_". (SEQUEST) init with SEQUEST nonspecific MS Instrument info: Manufacturer: UNKNOWN, Model: UNKNOWN, Ionization: UNKNOWN, Analyzer: UNKNOWN, Detector: UNKNOWN PeptideProphet (TPP v4.4 VUVUZELA (4.4.0 RC4) rev 0, Build 201008031534 (MinGW)) akel...@isb read in 149 1+, 3805 2+, 3949 3+, 99 4+, 10 5+, 4 6+, and 0 7+ spectra. Initialising statistical models ... Found 3671 Decoys, and 4345 Non-Decoys Iterations: .........10.........20... WARNING: Mixture model quality test failed for charge (1+). WARNING: Mixture model quality test failed for charge (7+). model complete after 24 iterations command completed in 2 sec running: "C:/Inetpub/tpp-bin/ProphetModels.pl -i interact.pep.xml -d R_" Analyzing interact.pep.xml ... Parsing search results "c:/Inetpub/wwwroot/ISB/data/ mito_p2_phospho_hetero_II_95ug_out (SEQUEST)"... => Total of 1115 hits. command completed in 0 sec running: "C:/Inetpub/tpp-bin/DatabaseParser "interact.pep.xml"" command completed in 0 sec running: "C:/Inetpub/tpp-bin/RefreshParser "interact.pep.xml" "C: \XCalibur\database\ipi_HUMAN_v3_74_withcontaminants_FRF"" - Searching the tree...error: could not open database file "c:/ XCalibur/database/ipi_HUMAN_v3_74_withcontaminants_FRF" (No such file or directory) - Building Commentz-Walter keyword tree... command "C:/Inetpub/tpp-bin/RefreshParser "interact.pep.xml" "C: \XCalibur\database\ipi_HUMAN_v3_74_withcontaminants_FRF"" failed: Unknown error command "C:/Inetpub/tpp-bin/RefreshParser "interact.pep.xml" "C: \XCalibur\database\ipi_HUMAN_v3_74_withcontaminants_FRF"" exited with non-zero exit code: -1 QUIT - the job is incomplete command "c:\Inetpub\tpp-bin\xinteract -Ninteract.pep.xml -p0.05 -l7 -O -dR_ c:/Inetpub/wwwroot/ISB/data/mito/mito_p2_phospho_hetero_II/ mito_p2_phospho_hetero_II_95ug_out.pep.xml" failed: Unknown error Command FAILED peptide prophet parameter --> Use decoy hits to pin down the negative distribution.Decoy Protein names begin with I use "R_" word , but my peptide prophet result include R_ protein(decoy hit protein) ... -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
