>From the output below, it looks like you do not have the gnuplot
software installed (the ProteinProphet output contains a line stating,
"sh: gnuplot: command not found " ).

Install gnuplot, try it again and ProteinProphet should work.

-Brian

On Aug 10, 6:23 pm, bio4647 <[email protected]> wrote:
> I' want to convert  CPAS pep.xml --> TPP protein prophet
>
> but error message
>
> ProteinProphet /usr/local/apache2/htdocs/data/test_2_interact.pep.xml /
> usr/local/apache2/htdocs/data/test_2_1_interact.prot.xml
> --------------------------------------------------------------------------------
>
> ProteinProphet (C++) by Insilicos LLC and LabKey Software, after the
> original Perl by A. Keller (TPP v4.3 JETSTREAM rev 1, Build
> 201008061922 (linux))
>  (xml input) (report Protein Length) (using degen pep info)
> . . . reading in /usr/local/apache2/htdocs/data/
> test_2_interact.pep.xml. . .
> . . . read in 0 1+, 53 2+, 0 3+, 8 4+, 0 5+, 0 6+, 0 7+ spectra with
> min prob 0.05
> sh: gnuplot: command not found
> INFO: mu=3.02439e-08, db_size=143584001
>
>  protein probabilities written to file /usr/local/apache2/htdocs/data/
> test_2_1_interact.prot.xml
>  direct your browser 
> tohttp://192.168.170.205//usr/local/apache2/htdocs/data/test_2_1_intera...
>
> command "gnuplot "/tmp/test_2_1_interact.prot.png.script.tmp.HxQaZB""
> failed with exit code 32512
> ProteinProphet: png file /usr/local/apache2/htdocs/data/
> test_2_1_interact.prot.png not written, error code 32512

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