Hi~
   I met the same problem recently. I used the Mascot and Xtandem
within TPP version 4.4 running on the windows XP
While I used the "Peptide analysis", the command line showed the error
message below:

"c:\Inetpub\tpp-bin\xinteract (TPP v4.4 VUVUZELA rev 1, Build
201010121551 (MinGW))

running: "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml" "c:/
Inetpub/wwwroot/ISB/data/file/filename.pep.xml" -L"7""
<PRE> Environment variable WEBSERVER_ROOT does not exist.

 For Windows users, you can set this environment variable
 through the Advanced tab under System Properties when you
 right-mouse-click on your My Computer icon.

 Set this environment variable to your webserver's document
 root directory such as c:\inetpub\wwwroot for IIS or
 c:\website\htdocs or WebSite Pro.

 Exiting.
command completed in 0 sec

running: "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml"
MINPROB=0.05"
fin: error opening interact.pep.xml

command "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml"
MINPROB=0.05" failed: Operation not permitted

command "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml"
MINPROB=0.05" exited with non-zero exit code: 1
QUIT - the job is incomplete

command "c:\Inetpub\tpp-bin\xinteract -Ninteract.pep.xml -p0.05 -l7 -O
c:/Inetpub/wwwroot/ISB/data/F001345testlocal/F001356.pep.xml" failed:
Operation not permitted

Command FAILED"

I have set the webserver root according to the indication of error
message. It still can't work.
Thank you for your help.

All bests

On 12月1日, 下午10時44分, catherine <[email protected]> wrote:
> Dear friends,
> This is the first time i post a message here.
>
> I was using the SEQUEST for database searching within TPP version
> 4.2.1 running on windowsXP, everything was fine until the pep.xml
> conversion step.  However, when I was trying to submit the 60 pep.xml
> files for peptideprophet, it always indicated an an "unknown error,
> the job is not completed". May I know what the problem is and how i
> can solve the problem?  Thanks a lot for the help and support!

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