XPRESS supports N15 metabolic labeling. I don't think ASAPRatio does but it's possible that functionality was added recently w/o my knowledge of it.
You're going to have to run 2 separate searches. One search will measure the normal (or "light") peptides; no modifications are specified here. The other search will measure the heavy peptides; statically modify each amino acid residue based on the number of nitrogens. You will have to do two separate XPRESS analysis for the light and heavy runs. Then combine together (say with ProteinProphet, feeding in the two separate PeptideProphet-ed runs). On Thu, Feb 17, 2011 at 12:17 PM, GATTACA <[email protected]> wrote: > Hello. > > A user wants to perform metabolic labelling (N15 labelling) on with > their sample. > I have two questions: > 1) Can TPP handle N15 metabolic labelling? > 2) How do you specify this modification in X!Tandem? > > Thanks in advance. > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To post to this group, send email to [email protected]. > To unsubscribe from this group, send email to > [email protected]. > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. > > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
