ASAPRatio has supported N15 for awhile. It does require a really long commandline, see this thread:
http://groups.google.com/group/spctools-discuss/browse_thread/thread/3be51d7bf8fa36cb/56c8d65c9105865d?lnk=gst&q=15N+ASAPRatio+Jake#56c8d65c9105865d -David On Thu, Feb 17, 2011 at 12:26 PM, Jimmy Eng <jke...@gmail.com> wrote: > XPRESS supports N15 metabolic labeling. I don't think ASAPRatio does > but it's possible that functionality was added recently w/o my > knowledge of it. > > You're going to have to run 2 separate searches. One search will > measure the normal (or "light") peptides; no modifications are > specified here. The other search will measure the heavy peptides; > statically modify each amino acid residue based on the number of > nitrogens. You will have to do two separate XPRESS analysis for the > light and heavy runs. Then combine together (say with ProteinProphet, > feeding in the two separate PeptideProphet-ed runs). > > > On Thu, Feb 17, 2011 at 12:17 PM, GATTACA <dfer...@umich.edu> wrote: >> Hello. >> >> A user wants to perform metabolic labelling (N15 labelling) on with >> their sample. >> I have two questions: >> 1) Can TPP handle N15 metabolic labelling? >> 2) How do you specify this modification in X!Tandem? >> >> Thanks in advance. >> >> -- >> You received this message because you are subscribed to the Google Groups >> "spctools-discuss" group. >> To post to this group, send email to spctools-discuss@googlegroups.com. >> To unsubscribe from this group, send email to >> spctools-discuss+unsubscr...@googlegroups.com. >> For more options, visit this group at >> http://groups.google.com/group/spctools-discuss?hl=en. >> >> > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To post to this group, send email to spctools-discuss@googlegroups.com. > To unsubscribe from this group, send email to > spctools-discuss+unsubscr...@googlegroups.com. > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. > > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to spctools-discuss@googlegroups.com. To unsubscribe from this group, send email to spctools-discuss+unsubscr...@googlegroups.com. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
