I'm stumped as I can't replicate the error.  Ruby sent me her data and
I had no problems running Out2XML to generate the pep.xml file.  Both
on a Windows box and under Linux.

- Jimmy

On Mar 1, 1:58 pm, Ruby <[email protected]> wrote:
> Hi-
> I just downloaded the latest version of TPP this week, so version:
> TPP v4.4 VUVUZELA rev 1, Build 201010121551 (MinGW)
>
> I'm still really hoping for an answer for the -Etrypsin error.....I'm
> stuck until I get that figured out.
>
> Thanks!!
> Ruby
>
> On Feb 28, 12:04 pm, Joe Slagel <[email protected]> wrote:
>
>
>
>
>
>
>
> > Hi Rudy,
>
> > For both questions you have, what version of TPP are you using? and I'm
> > assuming you are trying to convert the .RAW to mzXML using the petunia
> > interface?  (And I'll defer to others on your first question...unless you
> > don't get an answer).
>
> > -Joe
>
> > On Sat, Feb 26, 2011 at 6:02 PM, Ruby <[email protected]> wrote:
> > > Hi-
> > > 1) I'm trying to use the TPP to process my Sequest search results.
> > > (since I have access to sequest only on a cluster, I'm not able to use
> > > the TPP to setup the search, which is what I'd like to do). Rather,
> > > I'm trying to use the .out files for peptide & proteinprophet
> > > processing.
>
> > > However, I get this error when I try to process my directory that has
> > > the .out files from the Sequest search.
>
> > > command "c:\Inetpub\tpp-bin\Out2XML c:/Inetpub/wwwroot/ISB/data/
> > > RG_81_2 1 -Etrypsin" failed: Unknown error
>
> > > Is this due to the fact that I did a semi-enzyme search? How can I
> > > work around this? I didn't see a semi-enzyme choice in the 'options'
> > > section of the convert to pepXML page.
>
> > > 2) I'd like to do an XTandem search via the TPP. When I try to
> > > convert .RAW to mzXML, I get this error:
> > > 'c:\Inetpub\tpp-bin\ReAdW' is not recognized as an internal or
> > > external command, operable program or batch file.
>
> > > I thought that TPP wasn't using ReAdW anymore, but rather the
> > > MSConverter. I have downloaded the MSFileReader, but what parts of
> > > that go in the tpp-bin directory?? Basically, how do I get it
> > > configured so that it uses MSConvert for processing the .RAW files?
>
> > > Thanks!
> > > Ruby
>
> > > --
> > > You received this message because you are subscribed to the Google Groups
> > > "spctools-discuss" group.
> > > To post to this group, send email to [email protected].
> > > To unsubscribe from this group, send email to
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> > > For more options, visit this group at
> > >http://groups.google.com/group/spctools-discuss?hl=en.
>
> > --
> > Joe Slagel
> > Institute for Systems Biology
> > [email protected]
> > (206) 732-1362

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