Hi-
I just downloaded the latest version of TPP this week, so version:
TPP v4.4 VUVUZELA rev 1, Build 201010121551 (MinGW)

I'm still really hoping for an answer for the -Etrypsin error.....I'm
stuck until I get that figured out.

Thanks!!
Ruby

On Feb 28, 12:04 pm, Joe Slagel <[email protected]> wrote:
> Hi Rudy,
>
> For both questions you have, what version of TPP are you using? and I'm
> assuming you are trying to convert the .RAW to mzXML using the petunia
> interface?  (And I'll defer to others on your first question...unless you
> don't get an answer).
>
> -Joe
>
>
>
> On Sat, Feb 26, 2011 at 6:02 PM, Ruby <[email protected]> wrote:
> > Hi-
> > 1) I'm trying to use the TPP to process my Sequest search results.
> > (since I have access to sequest only on a cluster, I'm not able to use
> > the TPP to setup the search, which is what I'd like to do). Rather,
> > I'm trying to use the .out files for peptide & proteinprophet
> > processing.
>
> > However, I get this error when I try to process my directory that has
> > the .out files from the Sequest search.
>
> > command "c:\Inetpub\tpp-bin\Out2XML c:/Inetpub/wwwroot/ISB/data/
> > RG_81_2 1 -Etrypsin" failed: Unknown error
>
> > Is this due to the fact that I did a semi-enzyme search? How can I
> > work around this? I didn't see a semi-enzyme choice in the 'options'
> > section of the convert to pepXML page.
>
> > 2) I'd like to do an XTandem search via the TPP. When I try to
> > convert .RAW to mzXML, I get this error:
> > 'c:\Inetpub\tpp-bin\ReAdW' is not recognized as an internal or
> > external command, operable program or batch file.
>
> > I thought that TPP wasn't using ReAdW anymore, but rather the
> > MSConverter. I have downloaded the MSFileReader, but what parts of
> > that go in the tpp-bin directory?? Basically, how do I get it
> > configured so that it uses MSConvert for processing the .RAW files?
>
> > Thanks!
> > Ruby
>
> > --
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>
> --
> Joe Slagel
> Institute for Systems Biology
> [email protected]
> (206) 732-1362

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