Hi,
I came across this quote recently:
"PeptideProphet supplemented this parametric model with a variable
component mixture model and a semi-parametric model that incorporate
decoy database results...the Mascot implementation does not improve
discrimination and only uses the raw Mascot scores".

Is this true? In my experience, using Prophet's semi-parametric model
can significantly increase peptide sensitivity for Mascot results.
Thanks,
John

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