thank you for your help.
The TPP is latest version (TPP_Setup_v4_4_VUVUZELA_rev_1). the parameters that 
I used for SILAC search is quite simple. I selected: RUN PeptideProphet,Run 
ProteinProphet afterwards,RUN XPRESS,Change XPRESS residue mass difference (K 
8, R 10),RUN ASAPRatio,Change labeled residues to K, R..

It is quite surprise to see this error because I use the same parameters to run 
other similar files and it is fine. I could not figure out why there is error 
when I run these files. Sure I can send files to you. It is quite big and I do 
not know how I send to you. Do you only need raw files?

thank you again
Eileen
________________________________________
From: [email protected] [[email protected]] On 
Behalf Of Joseph Slagel [[email protected]]
Sent: Monday, September 19, 2011 2:48 PM
To: [email protected]
Subject: Re: [spctools-discuss] RE: ASAP Ratiopeptideparse.exe-application error

Eileen,

I'm afraid we'll need a little more information to help diagnosis the problem, 
such as the TPP version,  parameters you used and details about the files you 
are running it on.  Even better if you could share one of the files.

-Joe



On Mon, Sep 19, 2011 at 10:14 AM, Eileen Yue 
<[email protected]<mailto:[email protected]>> wrote:
Is there anyone who know this problem? I tried several times but all of them 
failed.

Thank you for your kind help
Eileen
________________________________________
From: 
[email protected]<mailto:[email protected]> 
[[email protected]<mailto:[email protected]>] 
On Behalf Of Eileen Yue [[email protected]<mailto:[email protected]>]
Sent: Friday, September 16, 2011 10:36 AM
To: [email protected]<mailto:[email protected]>
Subject: [spctools-discuss] ASAP Ratiopeptideparse.exe-application error

Dear all:
When I run the TPP for the SILAC analysis, during the ASAP analysis time, there 
is always one error showed up:

the instruction at "0x00406173" referenced memory at "0x7c35f048", the memory 
could not be "read"



Does anyone know what is this for and how do I fix this problem?

Thank you
Eileen

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