Hi Jimmy:
After I tested one fraction, I run TPP for my 12 fraction together to analyze
the xpress and Asap ratio. They run smoothly and completed after two days.
There is similar problem like before but without any error information: I
clicked the protein xpress ratio to see the all the peptide xpress ratio
information, they only showed:
<-- TPP v4.5 RAPTURE rev 0, Build 201109211427 (MinGW) -->
XPressCGIProteinDisplay (TPP v4.5 RAPTURE rev 0, Build 201109211427 (MinGW))
XPRESS ratio in ProteinProphet: light:heavy (1.29 ± 0.46)
sp|A0AV96|RBM47_HUMAN 6 entries used 1.29:1.00
1.00:0.77
--------------------------------------------------------------------------------c:/Inetpub/wwwroot/ISB/data/ISL1026/isl_er/interact.pep.xml
--------------------------------------------------------------------------------
Click RELOAD in your browser to re-evaluate ratio if changes have been made to
the XPRESS quantitation in any of the entries above. This will update what you
see in the current ratio just above.
To accept the current ratio back to ProteinProphet, click on the button below.
But when I checked the asap ratio, everything is fine.
Do you know how I solve this problem?
Thank you
Eileen
________________________________________
From: [email protected] [[email protected]] On
Behalf Of Eileen Yue [[email protected]]
Sent: Tuesday, October 18, 2011 3:22 PM
To: [email protected]
Subject: RE: [spctools-discuss] ASAP Ratiopeptideparse.exe-application error
Hi Jimmy:
Thank you so much and it works! I just tried one fraction and it works
perfectly!
So appreciate all of you for your kind help!
Eileen
________________________________________
From: [email protected] [[email protected]] On
Behalf Of Jimmy Eng [[email protected]]
Sent: Tuesday, October 18, 2011 1:41 PM
To: [email protected]
Subject: Re: [spctools-discuss] ASAP Ratiopeptideparse.exe-application error
Eileen,
Can you download the following executable and update your TPP binary
with this one:
http://proteomicsresource.washington.edu/dist/XPressPeptideUpdateParser.cgi
The files should go in c:\inetpub\tpp-bin\. Let me know if this fix
does not work for the problem you're experiencing.
- Jimmy
On Tue, Oct 18, 2011 at 10:51 AM, Eileen Yue <[email protected]> wrote:
> Hi Joe:
> I did convert mzml files. the problem is that the error showed up when I
> click the xpress ratio in prot.xml files. If I open the pep.shtml and click
> the xpress ratio, it is fine and showed me the chromatrography information
> for this peptide. This will not work if I open the prot.shtml and click the
> xpress ratio. The ASAP ratio is fine and I can open them and see the
> chromatography information.
>
> Thanks
> Eileen
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