Hi Dennis, This should not happen and it indicates a problem with running the algorithm on your dataset. Do you still see the message when you disable the option to force the distribution fit? This is an option that should be used for troubleshooting only and is not expected to generate good results. If you can package the dataset and post to our FTP server I can debug the issue further. Instructions for posting data to FTP can be found here: http://tools.proteomecenter.org/wiki/index.php?title=TPP:Frequently_Asked_Questions#How_do_I_upload_files_to_the_SPC_tools_team.3F
-David On Oct 3, 8:49 am, Dennis Goldfarb <[email protected]> wrote: > Hi, > > Every once in a while I receive an error stating: > > Iterations: .Assertion failed: idx < posProbs_->size(), file > Validation/InterProphet/InterProphetParser/KDModel.cxx, line 143 > > As far as I can tell this means that there aren't enough likely true spectra > to properly model the true and false distributions. Is this the correct way > to interpret the error? In the past I've only seen it when the # of decoy > hits is almost the same as forward hits, so that makes sense to me. Lately > I've had this error pop up when there are thousands more forward hits than > decoys, but only if I use the accurate mass binning model. The program > executes fine and the results look great (3000+ peptides of bait) without > accurate mass binning. We have an orbitrap and I haven't had this issue in > the past. Could this be a sign that our instrument's accuracy is slipping > and needs reconfiguration/maintenance? Could someone give me an idea of how > severely the accurate mass binning model penalizes the mass difference? > > I'm using TPP v4.5.0 on a Windows 7 Machine. I've tried combinations of > forcing the distribution fit, and using non-parametric distributions. > > Thank you, > > -- > Dennis Goldfarb > Graduate Student > Department of Computer Science > University of North Carolina at Chapel Hill -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
