Hi, Every once in a while I receive an error stating:
Iterations: .Assertion failed: idx < posProbs_->size(), file Validation/InterProphet/InterProphetParser/KDModel.cxx, line 143 As far as I can tell this means that there aren't enough likely true spectra to properly model the true and false distributions. Is this the correct way to interpret the error? In the past I've only seen it when the # of decoy hits is almost the same as forward hits, so that makes sense to me. Lately I've had this error pop up when there are thousands more forward hits than decoys, but only if I use the accurate mass binning model. The program executes fine and the results look great (3000+ peptides of bait) without accurate mass binning. We have an orbitrap and I haven't had this issue in the past. Could this be a sign that our instrument's accuracy is slipping and needs reconfiguration/maintenance? Could someone give me an idea of how severely the accurate mass binning model penalizes the mass difference? I'm using TPP v4.5.0 on a Windows 7 Machine. I've tried combinations of forcing the distribution fit, and using non-parametric distributions. Thank you, -- Dennis Goldfarb Graduate Student Department of Computer Science University of North Carolina at Chapel Hill -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
