I uploaded an example dataset with this issue. It is in /incoming/MAJOR_LAB_UNC_MA145.zip
On Mon, Oct 3, 2011 at 6:27 PM, SPCTools Admin <[email protected]>wrote: > Hi Dennis, > > This should not happen and it indicates a problem with running the > algorithm on your dataset. Do you still see the message when you > disable the option to force the distribution fit? This is an option > that should be used for troubleshooting only and is not expected to > generate good results. If you can package the dataset and post to our > FTP server I can debug the issue further. Instructions for posting > data to FTP can be found here: > > http://tools.proteomecenter.org/wiki/index.php?title=TPP:Frequently_Asked_Questions#How_do_I_upload_files_to_the_SPC_tools_team.3F > > -David > > > > On Oct 3, 8:49 am, Dennis Goldfarb <[email protected]> wrote: > > Hi, > > > > Every once in a while I receive an error stating: > > > > Iterations: .Assertion failed: idx < posProbs_->size(), file > > Validation/InterProphet/InterProphetParser/KDModel.cxx, line 143 > > > > As far as I can tell this means that there aren't enough likely true > spectra > > to properly model the true and false distributions. Is this the correct > way > > to interpret the error? In the past I've only seen it when the # of decoy > > hits is almost the same as forward hits, so that makes sense to me. > Lately > > I've had this error pop up when there are thousands more forward hits > than > > decoys, but only if I use the accurate mass binning model. The program > > executes fine and the results look great (3000+ peptides of bait) without > > accurate mass binning. We have an orbitrap and I haven't had this issue > in > > the past. Could this be a sign that our instrument's accuracy is slipping > > and needs reconfiguration/maintenance? Could someone give me an idea of > how > > severely the accurate mass binning model penalizes the mass difference? > > > > I'm using TPP v4.5.0 on a Windows 7 Machine. I've tried combinations of > > forcing the distribution fit, and using non-parametric distributions. > > > > Thank you, > > > > -- > > Dennis Goldfarb > > Graduate Student > > Department of Computer Science > > University of North Carolina at Chapel Hill > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To post to this group, send email to [email protected]. > To unsubscribe from this group, send email to > [email protected]. > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. > > -- Dennis Goldfarb Graduate Student Department of Computer Science University of North Carolina at Chapel Hill -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
