Thank you very much for the explanation!

Olga


On Nov 22, 5:45 pm, David Shteynberg
<[email protected]> wrote:
> Hi Olga,
>
> While the best iProphet probability is saved per spectrum (also the
> PeptideProphet probability) in the case of multiple searches this is done
> for completeness of the pepxml file. We want to show you the best ID for
> each spectrum you search.  However, when ProteinProphet runs in IPROPHET
> mode it considers each peptide sequence IDed in pepxml only once using the
> highest iProphet probability.
>
> -David
> On Nov 22, 2011 6:06 AM, "olgas" <[email protected]> wrote:
>
>
>
>
>
>
>
> > Dear all
>
> > In my iProphet output I get for each spectrum a separate iProphet
> > probability and I was wondering why, because in the iProphet paper it
> > says that "as the main outcome, iProphet permits the calculation of
> > more accurate posterior probabilities and FDR estimates at the level
> > of unique peptide sequences, where ONE probability is used for all
> > sequence identical PSMs observed [...]". What is now this one
> > probability? Is it just the highest of all the iProphet probabilities
> > for each peptide?
>
> > Many thanks in advance!
>
> > Olga
>
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