dear list,

i ran some tests with ptmprophetparser (tpp v4.5.1) on the output of xtandem, omssa and myrimatch after xinteract has been run on the original search engine output.
unfortunately, each test produced a different error:


> PTMProphetParser interact_myrimatch.pep.xml
Unknown file type. No file loaded.
Segmentation fault

> PTMProphetParser interact_tandem.pep.xml
....................................
....................................
....................................
(continues like that and does not stop even after 1h)

$ /cluster/apps/tpp/4.5.1/bin/PTMProphetParser interact_omssa.pep.xml
Failed to open input file '/cluster/work/scr3/quandtan/workflow/614/0/0/omssa/vagas_B1110_086.pep.xml.mzXML'.
Segmentation fault

it would be greatly appreciated if one of you, especially david ;-), could give me some advice on how to solve the problem. furthermore, i would like to kindly ask for help regading two other questions:
-  is ptmprophet is better applied after running xinteract or iprophet?
- what is the difference of specifying e.g. STY,80 as parameter or not specific modification at all?

many thanks in advance for any help from your side!

cheers,
andreas




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