I have been using the GLYC command in TPP 4.4.0 for more than a year with no problem. I recently upgraded to TPP 4.5.2 and can't seem to get the GLYC command to work for ProteinProphet. I have the "N- glycosylation data (color NXS/T)" option checked in petunia when doing ProteinProphet processing, and in the ProteinProphet header the "NxS/ T" box is checked. However, I do NOT see any pink highlighted N[115] in the peptides like I used to see nor does it calculate the # of glycopeptides at the bottom of the page - though most of my peptides do contain this modification. I am selecting the option to use the "Use N-glyc motif information" when I do the peptideprophet.
Is there something else I"m supposed to be doing to get this to work in the new update of TPP? Thanks Ruby -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
