Hi Rebekah,
This option is still listed as "experimental", but we will try to add
it to Petunia for the next release.  Your hunch to use the "-w" string
in the additional command-line options was on the right track;
unfortunately, this page uses another program to run ASAPRatio (called
xinteract), and thus the parameter format will not work for this case.

For the time being, your best option will be to run the tools up to
PeptideProphet via Petunia, then switch over to the command-line to
run ASAPRatioPeptideParser (with desired parameters) on the
interact.pep.xml file via the command-line.  To find out what
parameters to use, simply type "ASAPRatioPeptideParser " in the
command-line to get a usage statement.  You can then go back to
Petunia to run iProphet, ProteinProphet, etc after this is done.

Cheers,
--Luis


On Mon, Mar 5, 2012 at 1:46 PM, Ruby <[email protected]> wrote:
> Hi-
> I currently use ASAPRatio to quantify SILAC samples. I have TPP 4.5.2
> and would like to try WaveletQuant. I see in the instructions that it
> can be implemented by using ASAPRatioPeptideParser -w command line
> option. However, can I use the TPP via Petunia GUI to do this? I don't
> have experience using TPP via command line.  I tried typing in "-w"
> into the box under PeptideProphet options "Enter additional options to
> pass directly to the command-line (expert use only!)", but this didn't
> seem to do anything different.  I don't see a place under ASAPRatio
> Options for this.  Any help would be appreciated!
>
> Thanks!
> Rebekah
>
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