Hello, I am trying the newest TPP installed on Win7 using the installer. I have two small data sets of differentially labelled peptides searched separately with Tandem where one search specifies all light and the second all heavy labelled modifications. When running ASAPRatio, I select static modifications and tell it N-terminus & K are modified. It is pulling the correct mods from the tandem result file. But I get "error: cannot have variablel modified n in static mode."
This worked well with TPP 4.5.2, but now it isn't working. Is the new version of Tandem using different defaults for mods? I am not specifying any in the tandem.params file. I get the following error: (relevant part of pipeline output below) . . . running: "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser "TEST-HL-interact.pep.xml" -lnK -b -F -C -Z -r0.5 -S" K 170.142 176.18 n 43.0548 49.0928 error: cannot have variable modified n in static mode command "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser "TEST-HL-interact.pep.xml" -lnK -b -F -C -Z -r0.5 -S" failed: Operation not permitted command "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser "TEST-HL-interact.pep.xml" -lnK -b -F -C -Z -r0.5 -S" exited with non-zero exit code: 1 QUIT - the job is incomplete command "c:\Inetpub\tpp-bin\xinteract -NTEST-HL-interact.pep.xml -p0.05 -l6 -Op -I4 -I5 -A-lnK-b-F-C-Z-r0.5-S 120827_S-Aorta_GuanidineExtract-RABA-QC01.tandem.pep.xml c:/Inetpub/wwwroot/ISB/data/projects/S_Aorta/120827_Guanidine/light/120827_S-Aorta_GuanidineExtract-RABA-QC01.tandem.pep.xml" failed: Operation not permitted Thanks in advance for any help. Brian -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To view this discussion on the web visit https://groups.google.com/d/msg/spctools-discuss/-/Z0vbkWp9-l4J. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
