Hi Brian, Have you tried removing the static option -S when running this command?
Thanks, -David On Tue, Aug 28, 2012 at 2:50 PM, Brian Hampton <[email protected]> wrote: > Hello, > > I am trying the newest TPP installed on Win7 using the installer. I have > two small data sets of differentially labelled peptides searched separately > with Tandem where one search specifies all light and the second all heavy > labelled modifications. When running ASAPRatio, I select static > modifications and tell it N-terminus & K are modified. It is pulling the > correct mods from the tandem result file. But I get "error: cannot have > variablel modified n in static mode." > > This worked well with TPP 4.5.2, but now it isn't working. Is the new > version of Tandem using different defaults for mods? I am not specifying > any in the tandem.params file. I get the following error: (relevant part > of pipeline output below) > . > . > . > > running: "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser > "TEST-HL-interact.pep.xml" -lnK -b -F -C -Z -r0.5 -S" > K 170.142 176.18 > n 43.0548 49.0928 > error: cannot have variable modified n in static mode > > command "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser "TEST-HL-interact.pep.xml" > -lnK -b -F -C -Z -r0.5 -S" failed: Operation not permitted > > command "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser "TEST-HL-interact.pep.xml" > -lnK -b -F -C -Z -r0.5 -S" exited with non-zero exit code: 1 > QUIT - the job is incomplete > > command "c:\Inetpub\tpp-bin\xinteract -NTEST-HL-interact.pep.xml -p0.05 -l6 > -Op -I4 -I5 -A-lnK-b-F-C-Z-r0.5-S > 120827_S-Aorta_GuanidineExtract-RABA-QC01.tandem.pep.xml > c:/Inetpub/wwwroot/ISB/data/projects/S_Aorta/120827_Guanidine/light/120827_S-Aorta_GuanidineExtract-RABA-QC01.tandem.pep.xml" > failed: Operation not permitted > > > > Thanks in advance for any help. > > Brian > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To view this discussion on the web visit > https://groups.google.com/d/msg/spctools-discuss/-/Z0vbkWp9-l4J. > To post to this group, send email to [email protected]. > To unsubscribe from this group, send email to > [email protected]. > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
