Hey Brian, I am not sure what change broke your analysis and is causing the issue you have. To track this I will need to access your files and trace the code. Can you post your files somewhere I can get to them?
Thanks, -David On Fri, Aug 31, 2012 at 2:37 PM, Brian Hampton <[email protected]> wrote: > Nothing I have tried has allowed me to quantitate using ASAPRatio in > version 4.6 (Win7). So I switched back to 4.5.2 on Ubuntu 10.10 using the > same data set, database, and parameter files and I get nice quantitation. > > Here is the relevant part of the log from 4.5.2. > > running: "/usr/local/apps/tpp/bin/ASAPRatioPeptideParser > 'HL-interact.pep.xml.gz' -lnK -b -F -C -r0.5 -S" > K 170.142 176.18 > n 43.0548 49.0924 > static heavy label for file > /usr/local/apps/tpp/data/projects/S_Aorta/qc/H-120827_S-Aorta_GuanidineExtract-RABA-QC01.mzXML > static light label for file > /usr/local/apps/tpp/data/projects/S_Aorta/qc/L-120827_S-Aorta_GuanidineExtract-RABA-QC01.mzXML > command completed in 20 sec > > > In TPP 4.6, the two files are not read in at all and it throws an error > saying multiple modifications on n are not allowed and the analysis stops. > > > If anyone has successfully used ASAPRatio in TPP 4.6 to quantitate amine > reactive chemical labeling with stable isotopes I would like to hear how you > set up the pipeline to get ASAPRatio to actually work correctly. > > > Thanks in advance, > > > Brian > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To post to this group, send email to [email protected]. > To unsubscribe from this group, send email to > [email protected]. > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
