I guess, only Thermo may know exactly how to convert NCD into eV. I am not sure if the following docs from Thermo may really help, except for the units:
PSB 104. Normalized Collision Energy(TM) Technology https://static.thermoscientific.com/images/D13507~.pdf PSB 121. Enhanced Fragmentation of Small Molecules in a Thermo Scientific LTQ Series Linear Ion Trap Mass Spectrometer Using Stepped Normalized Collision Energy https://static.thermoscientific.com/images/D13514~.pdf Regards Dmitrii On Mon, Dec 17, 2012 at 1:56 PM, Matt Chambers <[email protected]>wrote: > Moving to proteowizard-support. > > Yes this "bug" is still there. I didn't know about NCD. What is NCD a > percent of? I.e. how can real electronvolts be calculated? > > Thanks for reporting, > -Matt > > > > On 12/14/2012 5:43 PM, Dmitrii Tchekhovskoi wrote: > >> Software: >> >> <software id="pwiz" version="3.0.3495"> >> <cvParam cvRef="MS" accession="MS:1000615" name="ProteoWizard" value=""/> >> >> Instrument: >> >> <cvParam cvRef="MS" accession="MS:1000556" name="LTQ Orbitrap XL" >> value=""/> >> >> Filter line: "ITMS + c NSI d Full ms2 [email protected] [210.00-1615.00]" >> >> Activation energy output: >> >> <cvParam cvRef="MS" accession="MS:1000133" name="collision-induced >> dissociation" value=""/> >> <cvParam cvRef="MS" accession="MS:1000045" name="collision energy" >> value="35.0" unitCvRef="UO" >> unitAccession="UO:0000266" unitName="electronvolt"/> >> >> *What is wrong: * >> >> >> in case of ITMS, 35.0 is a so called "Normalized collision energy" (NCD); >> it is measured in PERCENT >> (%), not eV. >> Therefore, unitName="electronvolt" is wrong. >> >> >> I am wondering if this bug is still in msconvert. >> >> Thank you in advance. >> >> Dmitrii >> > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
