I am trying to determine how to specify multiple variable modifications in the comet.params file.
I have stable isotope labeled primary amines so I need to specify 2 different mods on K and the N-terminus. Tandem uses [ to specify the N-terminus. What notation does Comet use? In the below example, I am guessing (probably incorrectly) "n" is used for denoting the N-terminus to Comet. Will this work? In Tandem, a similar approach would not work but there is a work around. For Comet, would I have to run two searches one for heavy and another for light using the "variable_N_terminus= " line in the comet.params for specifying the N-terminus? variable_mod1 = 15.9949 M 0 3 variable_mod2 = 42.04695 nK 0 3 variable_mod3 = 48.084606 nK 0 3 Thanks in advance for any help. Brian Brian Hampton Protein Analysis Lab Center for Vascular and Inflammatory Diseases University of Maryland School of Medicine 800 West Baltimore Street Rm 307 Baltimore MD 21201 V: 410-706-8207 F: 410-706-8234 -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at http://groups.google.com/group/spctools-discuss?hl=en. For more options, visit https://groups.google.com/groups/opt_out.
