Thank you Jimmy. Saves me much time tinkering knowing the approach won't work
Brian On Fri, Feb 22, 2013 at 11:14 AM, Jimmy Eng <[email protected]> wrote: > Brian, > > Will not work; there's currently no way to specify multiple variable mods > on the N-terminus so you'll have to manage with some work-around. There > are plans to treat the terminal mods the same way as amino acid mods in the > code (and this was a good reminder to go implement it) but it will be a > while before that version is available. > > > On Fri, Feb 22, 2013 at 2:39 AM, Brian Hampton <[email protected]>wrote: > >> I am trying to determine how to specify multiple variable modifications >> in the comet.params file. >> >> I have stable isotope labeled primary amines so I need to specify 2 >> different mods on K and the N-terminus. >> >> Tandem uses [ to specify the N-terminus. What notation does Comet use? >> >> In the below example, I am guessing (probably incorrectly) "n" is used >> for denoting the N-terminus to Comet. Will this work? In Tandem, a >> similar approach would not work but there is a work around. For Comet, >> would I have to run two searches one for heavy and another for light using >> the "variable_N_terminus= " line in the comet.params for specifying the >> N-terminus? >> >> variable_mod1 = 15.9949 M 0 3 >> variable_mod2 = 42.04695 nK 0 3 >> variable_mod3 = 48.084606 nK 0 3 >> >> >> Thanks in advance for any help. >> >> Brian >> >> Brian Hampton >> Protein Analysis Lab >> Center for Vascular and Inflammatory Diseases >> University of Maryland School of Medicine >> 800 West Baltimore Street Rm 307 >> Baltimore MD 21201 >> V: 410-706-8207 >> F: 410-706-8234 >> >> -- >> You received this message because you are subscribed to the Google Groups >> "spctools-discuss" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to [email protected]. >> To post to this group, send email to [email protected]. >> Visit this group at http://groups.google.com/group/spctools-discuss?hl=en >> . >> For more options, visit https://groups.google.com/groups/opt_out. >> >> >> > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to [email protected]. > To post to this group, send email to [email protected]. > Visit this group at http://groups.google.com/group/spctools-discuss?hl=en. > For more options, visit https://groups.google.com/groups/opt_out. > > > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at http://groups.google.com/group/spctools-discuss?hl=en. For more options, visit https://groups.google.com/groups/opt_out.
