Dear jimmy,

Hearty thanks for replying on my problem, yes there are both target and
decoy peptides available in 54ProtMix_S1R1.pep.xml file but still its
giving this error.

i have forwarded you the files please have a look on it .

regards,

puneet


On Sat, Sep 28, 2013 at 9:09 PM, Jimmy Eng <[email protected]> wrote:

> Are there peptide IDs in your 54ProtMix_S1R1.pep.xml file?  The error
> message is suggesting that there aren't any.  If you don't know how to
> tell, you can email me that pep.xml file and I'll take a look.
>
> - Jimmy
>
>
> On Fri, Sep 27, 2013 at 10:32 PM, Punit Kadimi <[email protected]>wrote:
>
>> Hello
>>
>> I tried to run peptide prophet on a Comet .pep.xml  output file but
>> peptide prophet is giving some error message.
>>
>> I am unable to figure out the problem.
>>
>> i am briefing out the process.
>>
>> (1) Searched a data set with comet.2012013.win64.exe using concatenated
>> database search option.
>> (2)  run peptide prophet "*C:\Inetpub\tpp-bin>PeptideProphetParser.exe
>> 54ProtMix_S1R1.pep.xml*"
>>
>> after going through both of the steps  it throws error message :
>>
>>  (Comet)
>> init with Comet Trypsin
>> MS Instrument info: Manufacturer: unknown, Model: unknown, Ionization:
>> UNKNOWN, Analyzer: UNKNOWN, Detector: UNKNOWN
>>
>>  PeptideProphet  (TPP v4.5 RAPTURE rev 2, Build 201202031108 (MinGW))
>> AKeller@ISB
>>  read in 0 1+, 0 2+, 0 3+, 0 4+, 0 5+, 0 6+, and 0 7+ spectra.
>>  read in no data.
>>
>> Can any one tell me please where i am going wrong .
>>
>> Thanks in advance
>>
>> Puneet
>>
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