Puneet,

https://groups.google.com/forum/#!topic/comet-ms/jUhIR5zWWcU

For Comet support, you need to be using TPP version 4.6.2 or newer.  The
current release is 4.6.3.  You're current running 4.5.2.  I just ran
PeptideProphetParser from version 4.6.3 on your file and it processed fine.

- Jimmy


On Fri, Sep 27, 2013 at 10:32 PM, Punit Kadimi <[email protected]> wrote:

> Hello
>
> I tried to run peptide prophet on a Comet .pep.xml  output file but
> peptide prophet is giving some error message.
>
> I am unable to figure out the problem.
>
> i am briefing out the process.
>
> (1) Searched a data set with comet.2012013.win64.exe using concatenated
> database search option.
> (2)  run peptide prophet "*C:\Inetpub\tpp-bin>PeptideProphetParser.exe
> 54ProtMix_S1R1.pep.xml*"
>
> after going through both of the steps  it throws error message :
>
>  (Comet)
> init with Comet Trypsin
> MS Instrument info: Manufacturer: unknown, Model: unknown, Ionization:
> UNKNOWN, Analyzer: UNKNOWN, Detector: UNKNOWN
>
>  PeptideProphet  (TPP v4.5 RAPTURE rev 2, Build 201202031108 (MinGW))
> AKeller@ISB
>  read in 0 1+, 0 2+, 0 3+, 0 4+, 0 5+, 0 6+, and 0 7+ spectra.
>  read in no data.
>
> Can any one tell me please where i am going wrong .
>
> Thanks in advance
>
> Puneet
>
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