Hello Peter,

Currently there is no way to do this in Petunia. You would have to perform
this step using command line.  Open the command line by going to start and
running the cmd command.  Once there use the cd command to change
directories to the directory containing your mzid files.  You will then
need to run the following command on the mzid files to fix the problems
with MSGF+ mzid format:


perl -pi -e 's/"\?"/"-"/'  MY_FILE.mzid


Now you can need to run the idconvert tool and rename the file:

idconvert MY_FILE.mzid --pepXML

mv MY_FILE.pepXML MY_FILE.pep.xml


Eventually this should become possible to do with  Petunia in an future
release.

Cheers,
-David




On Wed, Jul 8, 2015 at 9:55 PM, [email protected] <[email protected]> wrote:

> Hi,
>
>
> I want to use the TPP to combine, Mascot, X!Tandem and MSGF+ searches
> (amongst other things). I'm having trouble getting around some of the
> basics. I have successfully used the TPP in the past to process samples,
> but only with Mascot data.
>
>  Once I get my MSGF+ output files converted to pepXML I should be fine. I
> cannot work out how I get the MSGF+ files converted. The output generated
> by MSGF+ is an mzid file. I am also unclear as to where the database (with
> decoys) and mzXML files, used for searching, need to be located when
> generating this pepXML file. I have noticed error messages in the logs
> about accessing the database and mzXML file. I am generally using the
> Petunia interface as it's much nicer to work with multiple files.
>
>
>  Thanks,
>
>
>  Peter
>
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