Hello Jesse,
I think the issue is that ProteinProphet may not be finding the database used in the search. Can you run RefreshParser on the commandline as follows: RefreshParser c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/Kac.GluC.all.interact.pep.xml c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/Ecoli.fasta Then try to rerun ProteinProphet on the commandline ProteinProphet c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/Kac.GluC.all.interact.pep.xml c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/Kac.GluC.all.interact.prot.xml XML Let me know if you see any errors. Thank you, -David On Fri, Jul 24, 2015 at 2:19 PM, Joseph Slagel < [email protected]> wrote: > Jesse, > > Yes, I think David pinpointed an issue. It appears as if you downloaded > a developer alpha build of TPP. As an alpha build, its expected to have > lots of problems as it gets tested and they get fixed. I'd recommend > downloading the latest stable TPP release, 4.8.0, from sourceforge, > http://sourceforge.net/projects/sashimi/files/latest/download?source=files > > -Joe > > > On Fri, Jul 24, 2015 at 2:11 PM, David Shteynberg < > [email protected]> wrote: > >> Hi Jesse, >> >> This issue seems unrelated to the issue that I just corrected in >> InteractParser. >> >> It appears that on your system everything is installed to C:\TPP\bin >> whereas it should install to C:\Inetpub\tpp-bin. Are you perhaps Which >> version are you installing? Can you try this command in the commandline to >> see if it gives you any additional messages? >> >> Thank you, >> >> -David >> >> On Fri, Jul 24, 2015 at 1:15 PM, Jesse <[email protected]> wrote: >> >>> Hi David, >>> >>> Now I'm almost certain there is a problem with the installation on my >>> new systems. TPP wont even convert RAW files or download spectral >>> libraries. Any suggestions about where to start? >>> >>> *EXECUTING: C:/TPP/bin/msconvert >>> c:/Inetpub/wwwroot/ISB/data/dbase/OR20080317_S_SILAC-LH_1-1_01.RAW -v >>> --mzXML -o c:/Inetpub/wwwroot/ISB/data/dbase --filter "peakPicking true >>> [1,2]" * >>> >>> *Command FAILED* >>> RETURN CODE:13568 >>> >>> Best, >>> Jesse >>> >>> On Thursday, July 23, 2015 at 2:01:02 PM UTC-7, Jesse wrote: >>>> >>>> Hi David, >>>> >>>> I just sent you a link to the files with my new email address. I tried >>>> without the pI model and I got the same error. I suspect a problem with >>>> the network settings on the computers at work. >>>> >>>> Thanks for looking into this for me. >>>> >>>> Best, >>>> Jesse >>>> ------------------------------ >>>> *From:* [email protected] [ >>>> [email protected]] on behalf of David Shteynberg [ >>>> [email protected]] >>>> >>>> *Sent:* Thursday, July 23, 2015 11:58 AM >>>> *To:* spctools-discuss >>>> *Subject:* Re: [spctools-discuss] Re: Processing MSGF+ searches >>>> >>>> Hello Jesse, >>>> >>>> >>>> I am not sure that I can pinpoint the error without access to your >>>> file. Is this possible? Otherwise you can try the analysis with the >>>> following PeptideProphetParser options removing PI model which has not been >>>> extensively tested through MSGF+ and could be the culprit. >>>> >>>> PeptideProphetParser >>>> "C:/tmp/a03524/2015/Kac_GluC/test.interact.pep.xml" MINPROB=0.0 CLEVEL=2.5 >>>> PPM DECOYPROBS ACCMASS NONTT NONPARAM DECOY=XXX_ IGNORECHG=1 >>>> >>>> If this works then the problem is with the pI model. >>>> >>>> Let me know if you can forward me your test.interact.pep.xml file to >>>> test with. >>>> >>>> Thank you, >>>> -David >>>> >>>> On Wed, Jul 22, 2015 at 7:57 PM, Jesse <[email protected]> wrote: >>>> >>>>> Hi David, >>>>> >>>>> I'm having problems recently running PeptideProphet on MSGF+ search >>>>> output of GluC digests. I tried the perl script you suggest but I'm not >>>>> sure if it does anything because I'm not sure what it is supposed to do. >>>>> That line is meant to replace a single "\" with "-", correct? It seems to >>>>> start OK but then it quits. The GUI then prints return code: 65280. If >>>>> you could make a quick suggestion as to whether you think the problem is >>>>> my >>>>> pep.xml files again or my install I would greatly appreciate it. I tried >>>>> both the development release and 4.8. >>>>> >>>>> C:/TPP/bin/xinteract (TPP v5.0.0a2 Development, Build xxxx-xxxx (x86_64)) >>>>> PPM mode in Accurate Mass Model ... >>>>> PPM mode in Accurate Mass Model ... >>>>> >>>>> running: "C:/TPP/bin/InteractParser >>>>> "C:/tmp/a03524/2015/Kac_GluC/test.interact.pep.xml" >>>>> "150624_0002_CR1_GluC_Xyl_0p4_rep1b.pep.XML" >>>>> "150624_0003_CR1_GluC_Xyl_4_rep1.pep.XML" >>>>> "150624_0004_CR1_GluC_Glc_0p4_rep1.pep.XML" >>>>> "150624_0005_CR1_GluC_Glc_4_rep1.pep.XML" >>>>> "150624_0007_CR1_GluC_Xyl_0p4_rep2.pep.XML" >>>>> "150624_0008_CR1_GluC_Xyl_4_rep2.pep.XML" >>>>> "150624_0009_CR1_GluC_Glc_0p4_rep2.pep.XML" >>>>> "150624_0010_CR1_GluC_Glc_4_rep2.pep.XML" >>>>> "150624_0012_CR1_GluC_Xyl_0p4_rep3.pep.XML" >>>>> "150624_0014_CR1_GluC_Xyl_4_rep3.pep.XML" >>>>> "150624_0015_CR1_GluC_Glc_0p4_rep3.pep.XML" >>>>> "150624_0016_CR1_GluC_Glc_4_rep3.pep.XML" -L"7" -E"nonspecific"" >>>>> file 1: 150624_0002_CR1_GluC_Xyl_0p4_rep1b.pep.XMLWARNING: empty >>>>> raw_data in msms_run_summary tag ... trying mzML ... >>>>> Failed to open input file >>>>> 'c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0002_CR1_GluC_Xyl_0p4_rep1b.mzML'.WARNING: >>>>> cannot open data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0002_CR1_GluC_Xyl_0p4_rep1b.mzML >>>>> in msms_run_summary tag ...... trying .mzXML ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0002_CR1_GluC_Xyl_0p4_rep1b.mzXML >>>>> in msms_run_summary tag ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0002_CR1_GluC_Xyl_0p4_rep1b.mzXML >>>>> in msms_run_summary tag ... >>>>> file 2: 150624_0003_CR1_GluC_Xyl_4_rep1.pep.XMLWARNING: empty raw_data >>>>> in msms_run_summary tag ... trying mzML ... >>>>> Failed to open input file >>>>> 'c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0003_CR1_GluC_Xyl_4_rep1.mzML'.WARNING: >>>>> cannot open data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0003_CR1_GluC_Xyl_4_rep1.mzML >>>>> in msms_run_summary tag ...... trying .mzXML ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0003_CR1_GluC_Xyl_4_rep1.mzXML >>>>> in msms_run_summary tag ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0003_CR1_GluC_Xyl_4_rep1.mzXML >>>>> in msms_run_summary tag ... >>>>> file 3: 150624_0004_CR1_GluC_Glc_0p4_rep1.pep.XMLWARNING: empty raw_data >>>>> in msms_run_summary tag ... trying mzML ... >>>>> Failed to open input file >>>>> 'c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0004_CR1_GluC_Glc_0p4_rep1.mzML'.WARNING: >>>>> cannot open data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0004_CR1_GluC_Glc_0p4_rep1.mzML >>>>> in msms_run_summary tag ...... trying .mzXML ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0004_CR1_GluC_Glc_0p4_rep1.mzXML >>>>> in msms_run_summary tag ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0004_CR1_GluC_Glc_0p4_rep1.mzXML >>>>> in msms_run_summary tag ... >>>>> file 4: 150624_0005_CR1_GluC_Glc_4_rep1.pep.XMLWARNING: empty raw_data >>>>> in msms_run_summary tag ... trying mzML ... >>>>> Failed to open input file >>>>> 'c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0005_CR1_GluC_Glc_4_rep1.mzML'.WARNING: >>>>> cannot open data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0005_CR1_GluC_Glc_4_rep1.mzML >>>>> in msms_run_summary tag ...... trying .mzXML ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0005_CR1_GluC_Glc_4_rep1.mzXML >>>>> in msms_run_summary tag ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0005_CR1_GluC_Glc_4_rep1.mzXML >>>>> in msms_run_summary tag ... >>>>> file 5: 150624_0007_CR1_GluC_Xyl_0p4_rep2.pep.XMLWARNING: empty raw_data >>>>> in msms_run_summary tag ... trying mzML ... >>>>> Failed to open input file >>>>> 'c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0007_CR1_GluC_Xyl_0p4_rep2.mzML'.WARNING: >>>>> cannot open data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0007_CR1_GluC_Xyl_0p4_rep2.mzML >>>>> in msms_run_summary tag ...... trying .mzXML ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0007_CR1_GluC_Xyl_0p4_rep2.mzXML >>>>> in msms_run_summary tag ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0007_CR1_GluC_Xyl_0p4_rep2.mzXML >>>>> in msms_run_summary tag ... >>>>> file 6: 150624_0008_CR1_GluC_Xyl_4_rep2.pep.XMLWARNING: empty raw_data >>>>> in msms_run_summary tag ... trying mzML ... >>>>> Failed to open input file >>>>> 'c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0008_CR1_GluC_Xyl_4_rep2.mzML'.WARNING: >>>>> cannot open data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0008_CR1_GluC_Xyl_4_rep2.mzML >>>>> in msms_run_summary tag ...... trying .mzXML ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0008_CR1_GluC_Xyl_4_rep2.mzXML >>>>> in msms_run_summary tag ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0008_CR1_GluC_Xyl_4_rep2.mzXML >>>>> in msms_run_summary tag ... >>>>> file 7: 150624_0009_CR1_GluC_Glc_0p4_rep2.pep.XMLWARNING: empty raw_data >>>>> in msms_run_summary tag ... trying mzML ... >>>>> Failed to open input file >>>>> 'c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0009_CR1_GluC_Glc_0p4_rep2.mzML'.WARNING: >>>>> cannot open data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0009_CR1_GluC_Glc_0p4_rep2.mzML >>>>> in msms_run_summary tag ...... trying .mzXML ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0009_CR1_GluC_Glc_0p4_rep2.mzXML >>>>> in msms_run_summary tag ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0009_CR1_GluC_Glc_0p4_rep2.mzXML >>>>> in msms_run_summary tag ... >>>>> file 8: 150624_0010_CR1_GluC_Glc_4_rep2.pep.XMLWARNING: empty raw_data >>>>> in msms_run_summary tag ... trying mzML ... >>>>> Failed to open input file >>>>> 'c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0010_CR1_GluC_Glc_4_rep2.mzML'.WARNING: >>>>> cannot open data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0010_CR1_GluC_Glc_4_rep2.mzML >>>>> in msms_run_summary tag ...... trying .mzXML ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0010_CR1_GluC_Glc_4_rep2.mzXML >>>>> in msms_run_summary tag ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0010_CR1_GluC_Glc_4_rep2.mzXML >>>>> in msms_run_summary tag ... >>>>> file 9: 150624_0012_CR1_GluC_Xyl_0p4_rep3.pep.XMLWARNING: empty raw_data >>>>> in msms_run_summary tag ... trying mzML ... >>>>> Failed to open input file >>>>> 'c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0012_CR1_GluC_Xyl_0p4_rep3.mzML'.WARNING: >>>>> cannot open data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0012_CR1_GluC_Xyl_0p4_rep3.mzML >>>>> in msms_run_summary tag ...... trying .mzXML ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0012_CR1_GluC_Xyl_0p4_rep3.mzXML >>>>> in msms_run_summary tag ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0012_CR1_GluC_Xyl_0p4_rep3.mzXML >>>>> in msms_run_summary tag ... >>>>> file 10: 150624_0014_CR1_GluC_Xyl_4_rep3.pep.XMLWARNING: empty raw_data >>>>> in msms_run_summary tag ... trying mzML ... >>>>> Failed to open input file >>>>> 'c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0014_CR1_GluC_Xyl_4_rep3.mzML'.WARNING: >>>>> cannot open data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0014_CR1_GluC_Xyl_4_rep3.mzML >>>>> in msms_run_summary tag ...... trying .mzXML ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0014_CR1_GluC_Xyl_4_rep3.mzXML >>>>> in msms_run_summary tag ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0014_CR1_GluC_Xyl_4_rep3.mzXML >>>>> in msms_run_summary tag ... >>>>> file 11: 150624_0015_CR1_GluC_Glc_0p4_rep3.pep.XMLWARNING: empty >>>>> raw_data in msms_run_summary tag ... trying mzML ... >>>>> Failed to open input file >>>>> 'c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0015_CR1_GluC_Glc_0p4_rep3.mzML'.WARNING: >>>>> cannot open data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0015_CR1_GluC_Glc_0p4_rep3.mzML >>>>> in msms_run_summary tag ...... trying .mzXML ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0015_CR1_GluC_Glc_0p4_rep3.mzXML >>>>> in msms_run_summary tag ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0015_CR1_GluC_Glc_0p4_rep3.mzXML >>>>> in msms_run_summary tag ... >>>>> file 12: 150624_0016_CR1_GluC_Glc_4_rep3.pep.XMLWARNING: empty raw_data >>>>> in msms_run_summary tag ... trying mzML ... >>>>> Failed to open input file >>>>> 'c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0016_CR1_GluC_Glc_4_rep3.mzML'.WARNING: >>>>> cannot open data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0016_CR1_GluC_Glc_4_rep3.mzML >>>>> in msms_run_summary tag ...... trying .mzXML ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0016_CR1_GluC_Glc_4_rep3.mzXML >>>>> in msms_run_summary tag ... >>>>> SUCCESS: CORRECTED data file >>>>> c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/150624_0016_CR1_GluC_Glc_4_rep3.mzXML >>>>> in msms_run_summary tag ... >>>>> processed altogether 186504 results >>>>> INFO: Results written to file: >>>>> C:/tmp/a03524/2015/Kac_GluC/test.interact.pep.xml >>>>> command completed in 86 sec >>>>> >>>>> running: "C:/TPP/bin/DatabaseParser >>>>> "C:/tmp/a03524/2015/Kac_GluC/test.interact.pep.xml"" >>>>> command completed in 2 sec >>>>> >>>>> running: "C:/TPP/bin/RefreshParser >>>>> "C:/tmp/a03524/2015/Kac_GluC/test.interact.pep.xml" >>>>> "C:\Jesse\Ecoli.fasta"" >>>>> - Searching the tree...opening "c:/Jesse/Ecoli.fasta" as >>>>> "c:/Inetpub/wwwroot/ISB/data/2015/Kac_GluC/Ecoli.fasta" >>>>> >>>>> - Linking duplicate entries... - Printing results... >>>>> >>>>> - Building Commentz-Walter keyword tree...command completed in 22 sec >>>>> >>>>> running: "C:/TPP/bin/PeptideProphetParser >>>>> "C:/tmp/a03524/2015/Kac_GluC/test.interact.pep.xml" MINPROB=0.05 >>>>> CLEVEL=2.5 PPM PPM ACCMASS PI NONTT NONPARAM DECOY=XXX_ IGNORECHG=1" >>>>> using Accurate Mass Bins >>>>> using PPM mass difference >>>>> using pI >>>>> Using Decoy Label "XXX_". >>>>> Ignoring charge 1+ spectra. >>>>> Not using ntt model >>>>> Using non-parametric distributions >>>>> (MS-GF+)WARNING: Support of MSGF+ may not be full. There exist known >>>>> issues with the way MSGF+ encodes certain modifications in pep.xml that >>>>> may not be correct. Also, high-scoring DECOY have been observed in MSGF+ >>>>> analysis. The user is encouraged to be vigilant in comparing model >>>>> estimated error-rates to the DECOY-estimated error-rates to make sure the >>>>> two agree and set CLEVEL parameter accordingly: see >>>>> http://tools.proteomecenter.org/wiki/index.php?title=TPP:Frequently_Asked_Questions#What_is_CLEVEL_and_when_do_I_use_it.3F >>>>> adding Accurate Mass mixture distr >>>>> adding pI mixture distr >>>>> init with MS-GF+ nonspecific >>>>> >>>>> command "C:/TPP/bin/PeptideProphetParser >>>>> "C:/tmp/a03524/2015/Kac_GluC/test.interact.pep.xml" MINPROB=0.05 >>>>> CLEVEL=2.5 PPM PPM ACCMASS PI NONTT NONPARAM DECOY=XXX_ IGNORECHG=1" >>>>> failed: Unknown error >>>>> >>>>> command "C:/TPP/bin/PeptideProphetParser >>>>> "C:/tmp/a03524/2015/Kac_GluC/test.interact.pep.xml" MINPROB=0.05 >>>>> CLEVEL=2.5 PPM PPM ACCMASS PI NONTT NONPARAM DECOY=XXX_ IGNORECHG=1" >>>>> exited with non-zero exit code: 255 >>>>> QUIT - the job is incomplete >>>>> >>>>> >>>>> On Thursday, July 9, 2015 at 2:33:16 PM UTC-7, [email protected] wrote: >>>>>> >>>>>> Hi David, >>>>>> >>>>>> I can cope with the command line. Thanks for the info I never would >>>>>> have worked that one out... >>>>>> >>>>>> Peter >>>>>> >>>>>> On Thursday, July 9, 2015 at 2:55:41 PM UTC+10, [email protected] >>>>>> wrote: >>>>>>> >>>>>>> Hi, >>>>>>> >>>>>>> >>>>>>> I want to use the TPP to combine, Mascot, X!Tandem and MSGF+ >>>>>>> searches (amongst other things). I'm having trouble getting around some >>>>>>> of >>>>>>> the basics. I have successfully used the TPP in the past to process >>>>>>> samples, but only with Mascot data. >>>>>>> >>>>>>> Once I get my MSGF+ output files converted to pepXML I should be >>>>>>> fine. I cannot work out how I get the MSGF+ files converted. The output >>>>>>> generated by MSGF+ is an mzid file. I am also unclear as to where the >>>>>>> database (with decoys) and mzXML files, used for searching, need to be >>>>>>> located when generating this pepXML file. I have noticed error messages >>>>>>> in >>>>>>> the logs about accessing the database and mzXML file. I am generally >>>>>>> using >>>>>>> the Petunia interface as it's much nicer to work with multiple files. >>>>>>> >>>>>>> >>>>>>> Thanks, >>>>>>> >>>>>>> >>>>>>> Peter >>>>>>> >>>>>> -- >>>>> You received this message because you are subscribed to the Google >>>>> Groups "spctools-discuss" group. >>>>> To unsubscribe from this group and stop receiving emails from it, send >>>>> an email to [email protected]. >>>>> To post to this group, send email to [email protected] >>>>> . >>>>> Visit this group at http://groups.google.com/group/spctools-discuss. >>>>> For more options, visit https://groups.google.com/d/optout. >>>>> >>>> >>>> -- >>>> You received this message because you are subscribed to the Google >>>> Groups "spctools-discuss" group. >>>> To unsubscribe from this group and stop receiving emails from it, send >>>> an email to [email protected]. >>>> To post to this group, send email to [email protected]. >>>> Visit this group at http://groups.google.com/group/spctools-discuss. >>>> For more options, visit https://groups.google.com/d/optout. >>>> >>> -- >>> You received this message because you are subscribed to the Google >>> Groups "spctools-discuss" group. >>> To unsubscribe from this group and stop receiving emails from it, send >>> an email to [email protected]. >>> To post to this group, send email to [email protected]. >>> Visit this group at http://groups.google.com/group/spctools-discuss. >>> For more options, visit https://groups.google.com/d/optout. >>> >> >> -- >> You received this message because you are subscribed to the Google Groups >> "spctools-discuss" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to [email protected]. >> To post to this group, send email to [email protected]. >> Visit this group at http://groups.google.com/group/spctools-discuss. >> For more options, visit https://groups.google.com/d/optout. >> > > > > -- > Joe Slagel > Institute for Systems Biology > [email protected] > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to [email protected]. > To post to this group, send email to [email protected]. > Visit this group at http://groups.google.com/group/spctools-discuss. > For more options, visit https://groups.google.com/d/optout. > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at http://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
