hello

I try to create spectra library through spectrast, got the same error too. 
And I download the TPP 5.0.0 and run it on windows10, but the same error 
occured.

how can I solve this problem?

yuderui

在 2014年11月29日星期六 UTC+8上午9:04:12,Eric Deutsch写道:
>
> If you compile on Linux, your executable will almost surely be 64-bit as 
> all modern distros are 64-bit.
>
>  
>
> The issue with Windows is that our default compiler is still 32-bit and so 
> we generate 32-bits executables. But we’re working on creating a 64-bit 
> Windows build, but it is a little tricky. However, we do have a valid 
> 64-bit executable for SpectraST that I pointed you to. For the very reason 
> that you encountered. Starting with TPP 5.0, everything will be 64-bit.
>
>  
>
> Regards,
>
> Eric
>
>  
>
>  
>
> *From:* [email protected] <javascript:> [mailto:
> [email protected] <javascript:>] *On Behalf Of *delphine wood
> *Sent:* Wednesday, November 26, 2014 3:25 AM
> *To:* [email protected] <javascript:>
> *Subject:* Re: [spctools-discuss] spectraST error
>
>  
>
> Hello,
>
>  
>
> I tried to run the spectrast search on the big human_nist_protein library 
> on a 64 bit windows, but with the actual version of TPP, and as expected 
> the same error occured. Thus I installed TPP on linux, and it seems to be 
> working; at least the same error doesn't occur. I didn't tried to use the 
> 64-bit 
> executable for SpectraST, unless it is included in the sources of the 
> last version of tpp I used for the linux installation...
>
> Delphine
>
>  
>
>  
>
> 2014-11-20 18:20 GMT+01:00 Eric Deutsch <[email protected] 
> <javascript:>>:
>
> In addition to running on a 64-bit operating system, you will need a 
> 64-bit SpectraST executable. I think there is a test 64-bit executable for 
> SpectraST at:
>
>  
>
>
> http://sourceforge.net/projects/sashimi/files/Trans-Proteomic%20Pipeline%20%28TPP%29/TPP%20v0.0%20%28Development%29/
>
>  
>
> you can download the “spectrast.exe” file there and replace the installed 
> spectrast.exe with the one that you download.
>
>  
>
> That may fix the problem.
>
>  
>
> Eric
>
>  
>
>  
>
> *From:* [email protected] <javascript:> [mailto:
> [email protected] <javascript:>] *On Behalf Of *David 
> Shteynberg
> *Sent:* Thursday, November 20, 2014 8:20 AM
> *To:* spctools-discuss
> *Subject:* Re: [spctools-discuss] spectraST error
>
>  
>
> You will also require a 64bit TPP build for windows that we are still 
> working on.
>
> David
>
> On Nov 20, 2014 8:12 AM, "delphine wood" <[email protected] <javascript:>> 
> wrote:
>
> I have read the issue note( release notes ). 
> <http://tools.proteomecenter.org/wiki/index.php?title=TPP:4.7.1_Release_Notes>
>
> Do you think that if I install TPP on a windows 7 , 64 bit system the 
> problem could be solved? I will try and let you know if this solved the 
> problem.
>
> Delphine
>
>  
>
> 2014-11-20 11:49 GMT+01:00 Rene B <[email protected] <javascript:>>:
>
> I tried this as well a few weeks ago with several human libraries, and ran 
> in the same issue. I assumed it was a windows memory issue, the NIST human 
> libraries are over 2GB. This is a known issue listed in the TPP4.7.1 release 
> notes 
> <http://tools.proteomecenter.org/wiki/index.php?title=TPP:4.7.1_Release_Notes>.
>  
> I have not tried it on linux though.
>
>  
>
> Rene
>
> On Thursday, 20 November 2014 11:26:49 UTC+1, Delphine wrote:
>
> I downloaded it again but the same error remains... I tried with the 
> HumanPlasma_2012-08_all.splib library and it works, but it's not the 
> library I'm interested in.... Could it be that the 
> NIST_human_IT_2010_01_14_7AA.splib is corrupted from the source?
>
> Delphine
>
>  
>
> 2014-11-18 19:15 GMT+01:00 David Shteynberg <
> [email protected]>:
>
> Looks like this file
>
> "c:/Inetpub/wwwroot/ISB/data/dbase/speclibs/NIST_human_IT_2010_01_14_7AA.splib"
>
> is corrupted.  Can you try to download the file again?
>
> On Tue, Nov 18, 2014 at 9:52 AM, Delphine <[email protected]> wrote:
> > Hello,
> >
> > I'm trying to use spectraST. I downloaded the
> > NIST_human_IT_2010_01_14_7AA.splib library in a "dbase/speclibs/" folder,
> > with the tpp tool and also directly from the website. Now whatever I do 
> with
> > this library (interrogation or just making a consensus library) I get 
> this
> > error:
> >
> >
> > SpectraST started at Tue Nov 18 18:06:47 2014.
> > Creating CONSENSUS library from
> > 
> "c:/Inetpub/wwwroot/ISB/data/dbase/speclibs/NIST_human_IT_2010_01_14_7AA.splib"
> > Importing
> > 
> ions...500...1000...1500...2000...2500...3000...3500...4000...4500...5000...5500...6000...6500...7000...7500...800
> >         ==== FATAL ERROR. Exiting immediately. ====
> >       Error trace :
> >       GENERAL: Corrupt .splib file from which to import entry.
> >       ===========================================
> >
> > Could someone help me solving this problem?
> >
> > Thanks
> >
> > Delphine
> >
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