Hello, I tried to run the spectrast search on the big human_nist_protein library on a 64 bit windows, but with the actual version of TPP, and as expected the same error occured. Thus I installed TPP on linux, and it seems to be working; at least the same error doesn't occur. I didn't tried to use the 64-bit executable for SpectraST, unless it is included in the sources of the last version of tpp I used for the linux installation...
Delphine 2014-11-20 18:20 GMT+01:00 Eric Deutsch <[email protected]>: > In addition to running on a 64-bit operating system, you will need a > 64-bit SpectraST executable. I think there is a test 64-bit executable for > SpectraST at: > > > > > http://sourceforge.net/projects/sashimi/files/Trans-Proteomic%20Pipeline%20%28TPP%29/TPP%20v0.0%20%28Development%29/ > > > > you can download the “spectrast.exe” file there and replace the installed > spectrast.exe with the one that you download. > > > > That may fix the problem. > > > > Eric > > > > > > *From:* [email protected] [mailto: > [email protected]] *On Behalf Of *David Shteynberg > *Sent:* Thursday, November 20, 2014 8:20 AM > *To:* spctools-discuss > *Subject:* Re: [spctools-discuss] spectraST error > > > > You will also require a 64bit TPP build for windows that we are still > working on. > > David > > On Nov 20, 2014 8:12 AM, "delphine wood" <[email protected]> wrote: > > I have read the issue note( release notes ). > <http://tools.proteomecenter.org/wiki/index.php?title=TPP:4.7.1_Release_Notes> > > Do you think that if I install TPP on a windows 7 , 64 bit system the > problem could be solved? I will try and let you know if this solved the > problem. > > Delphine > > > > 2014-11-20 11:49 GMT+01:00 Rene B <[email protected]>: > > I tried this as well a few weeks ago with several human libraries, and ran > in the same issue. I assumed it was a windows memory issue, the NIST human > libraries are over 2GB. This is a known issue listed in the TPP4.7.1 release > notes > <http://tools.proteomecenter.org/wiki/index.php?title=TPP:4.7.1_Release_Notes>. > I have not tried it on linux though. > > > > Rene > > On Thursday, 20 November 2014 11:26:49 UTC+1, Delphine wrote: > > I downloaded it again but the same error remains... I tried with the > HumanPlasma_2012-08_all.splib library and it works, but it's not the > library I'm interested in.... Could it be that the > NIST_human_IT_2010_01_14_7AA.splib is corrupted from the source? > > Delphine > > > > 2014-11-18 19:15 GMT+01:00 David Shteynberg < > [email protected]>: > > Looks like this file > > "c:/Inetpub/wwwroot/ISB/data/dbase/speclibs/NIST_human_IT_2010_01_14_7AA.splib" > > is corrupted. Can you try to download the file again? > > On Tue, Nov 18, 2014 at 9:52 AM, Delphine <[email protected]> wrote: > > Hello, > > > > I'm trying to use spectraST. I downloaded the > > NIST_human_IT_2010_01_14_7AA.splib library in a "dbase/speclibs/" folder, > > with the tpp tool and also directly from the website. Now whatever I do > with > > this library (interrogation or just making a consensus library) I get > this > > error: > > > > > > SpectraST started at Tue Nov 18 18:06:47 2014. > > Creating CONSENSUS library from > > > "c:/Inetpub/wwwroot/ISB/data/dbase/speclibs/NIST_human_IT_2010_01_14_7AA.splib" > > Importing > > > ions...500...1000...1500...2000...2500...3000...3500...4000...4500...5000...5500...6000...6500...7000...7500...800 > > ==== FATAL ERROR. Exiting immediately. ==== > > Error trace : > > GENERAL: Corrupt .splib file from which to import entry. > > =========================================== > > > > Could someone help me solving this problem? > > > > Thanks > > > > Delphine > > > > -- > > You received this message because you are subscribed to the Google Groups > > "spctools-discuss" group. > > To unsubscribe from this group and stop receiving emails from it, send an > > > email to [email protected]. > > To post to this group, send email to [email protected]. > > Visit this group at http://groups.google.com/group/spctools-discuss. > > For more options, visit https://groups.google.com/d/optout. > > -- > You received this message because you are subscribed to a topic in the > Google Groups "spctools-discuss" group. > To unsubscribe from this topic, visit > https://groups.google.com/d/topic/spctools-discuss/R_oTf7Bdqj8/unsubscribe > . > To unsubscribe from this group and all its topics, send an email to > [email protected]. > To post to this group, send email to [email protected]. > Visit this group at http://groups.google.com/group/spctools-discuss. > For more options, visit https://groups.google.com/d/optout. > > > > -- > You received this message because you are subscribed to a topic in the > Google Groups "spctools-discuss" group. > To unsubscribe from this topic, visit > https://groups.google.com/d/topic/spctools-discuss/R_oTf7Bdqj8/unsubscribe > . > To unsubscribe from this group and all its topics, send an email to > [email protected]. > To post to this group, send email to [email protected]. > Visit this group at http://groups.google.com/group/spctools-discuss. > For more options, visit https://groups.google.com/d/optout. > > > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to [email protected]. > To post to this group, send email to [email protected]. > Visit this group at http://groups.google.com/group/spctools-discuss. > For more options, visit https://groups.google.com/d/optout. > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to [email protected]. > To post to this group, send email to [email protected]. > Visit this group at http://groups.google.com/group/spctools-discuss. > For more options, visit https://groups.google.com/d/optout. > > -- > You received this message because you are subscribed to a topic in the > Google Groups "spctools-discuss" group. > To unsubscribe from this topic, visit > https://groups.google.com/d/topic/spctools-discuss/R_oTf7Bdqj8/unsubscribe > . > To unsubscribe from this group and all its topics, send an email to > [email protected]. > To post to this group, send email to [email protected]. > Visit this group at http://groups.google.com/group/spctools-discuss. > For more options, visit https://groups.google.com/d/optout. > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. 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