Hi Yaarub, When you combine the results of multiple searches in iProphet each search must first be analyzed independently using PeptideProphet. Then the PeptideProphet results (interact.pep.xml by default) from each of the search engines must be input to iProphet on the refine/combine analysis tab in the Petunia user interface. iProphet will create one file picking the highest scoring result for each spectrum among the search engines applied.
-David On Mon, May 22, 2017 at 2:03 AM, Yaarub Musa <[email protected]> wrote: > Hello Everyone, > > I have an issue with iProphet and I would be grateful if someone could > help as I am new to TPP. > > I am trying running both tandem and comet search engine using QExactive > Plus-acquired data and but I have been facing an issue with iProphet as the > process fails. > > I have repeated the process with tutorial data (Shubert et al., 2015) > where the data were acquired using TripleToF 5600 and all processes went > through successfully. > So I believe a certain parameter in my search is not compatible with > iProphet ( I had to change some parameters as my data were acquired on > QExactive). > > the iProphet process log is attached to this thread. I would appreciate > any help. > > best regards, > Yaarub > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to [email protected]. > To post to this group, send email to [email protected]. > Visit this group at https://groups.google.com/group/spctools-discuss. > For more options, visit https://groups.google.com/d/optout. > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
