Hi Luis, I just took a look at the quantitation.tsv file. Interestingly enough for the protein I am looking at there is only one peptide listed in this table, however, when looking at the ipro.pep.xml file for peptides of this protein I have eleven of them, all with ipro > 0.99 and some with intensities of 0 in the competitive inhibitor-treated channel...not sure what to make of this. Other proteins are listed with peptides and have the yes/no indicator for "kept?" and show intensities, in some cases zero... Are there other parameters that are preventing these peptides from showing up in the quantitation.tsv file?
Best, Adam On Friday, November 10, 2017 at 2:58:08 PM UTC-6, Luis wrote: > > > I forgot to add that once the protein quantitation is done, Libra will > also produce a "quantitation.tsv" file that you can use downstream for > re-evaluating protein ratios with any statistical(s) test you wish -- say > in R, Excel, etc. The file structure is fairly straightforward: protein > ratios and errors, followed by those of its peptides, including adjusted > intensities and a kept flag (used/not used in quant). > > ---Luis > > > On Fri, Nov 10, 2017 at 12:49 PM, Adam R <[email protected] <javascript:>> > wrote: > >> Thanks Luis for the response! >> >> Adam >> >> On Friday, November 10, 2017 at 2:30:33 PM UTC-6, Luis wrote: >>> >>> Hi Adam, >>> >>> We have plans to add some of these features to Libra as well as others, >>> but none are ready at the moment. >>> >>> One thing you could try is to select to normalize against the sum of the >>> reagent profiles (instead of selecting a channel to quantify against) -- >>> this should give you a value for all channels, even when some are missing. >>> >>> Cheers, >>> --Luis >>> >>> >>> On Fri, Nov 10, 2017 at 11:44 AM, Adam R <[email protected]> wrote: >>> >>>> Is there a way to retain quantified peptides for the protein quant with >>>> Libra? I understand that Libra discards peptides without all >>>> channels...what about in instances where the you expect there to be little >>>> or no intensity? I have data from pulldowns where we don't expect to see >>>> much of the protein in the presence of a competitive inhibitor, so in this >>>> case it would be of use to retain such quants where the intensity is zero >>>> in the presence of inhibitor but several thousands in the pulldown >>>> channel...Is there a way to toggle on/off the peptides that are used for >>>> the protein quant in Libra? I'm thinking along the lines like in Xpress >>>> where you can toggle whether or not a peptide is used for the protein >>>> quant. Is there also a way to modify the number of standard deviations >>>> that >>>> are used to discard a peptide from being used in the protein quant? >>>> >>>> Best, >>>> >>>> Adam >>>> >>>> -- >>>> You received this message because you are subscribed to the Google >>>> Groups "spctools-discuss" group. >>>> To unsubscribe from this group and stop receiving emails from it, send >>>> an email to [email protected]. >>>> To post to this group, send email to [email protected]. >>>> Visit this group at https://groups.google.com/group/spctools-discuss. >>>> For more options, visit https://groups.google.com/d/optout. >>>> >>> >>> -- >> You received this message because you are subscribed to the Google Groups >> "spctools-discuss" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to [email protected] <javascript:>. >> To post to this group, send email to [email protected] >> <javascript:>. >> Visit this group at https://groups.google.com/group/spctools-discuss. >> For more options, visit https://groups.google.com/d/optout. >> > > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
