Hi Oded,  Which release candidate are you referring to?  The earlier
candidates may have a bug that is corrected in a later version. If you can
share some data and specifics about the missing PSMs I can run it here and
troubleshoot the problem.

Thanks!

David

P.S. I am on vacation this week so will troubleshoot next week.

On Mon, Apr 12, 2021, 11:49 AM Oded <[email protected]> wrote:

> Hi there,
> We recently download the TPP 6.0 RC and while using it we noticed that we
> obtain fewer peptides IDs than what we got for the same dataset and search
> output with version 5.2.
> Many of the missing peptides seem to have decent Expect value and MS/MS
> following a visual inspection.
> This seems that this is due to changes in PeptideProphet and are not
> related to the search itself that was done with Comet.
> Is there any additional parameter that should be included in the new
> version in order to restore the missing peptides?
> Many thanks,
> Oded
>
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