I don't mind taking a crack at it over vacation.   Please let me know where
I can pull the data from. I might not have quick solution for you but I can
get started looking for the problem.  Which search engine did you use
here?   I would need mzML data search results and the fasta database to get
started.

Thanks!



Thanks!

On Mon, Apr 12, 2021, 1:38 PM Oded <[email protected]> wrote:

> Hi David,
> Thank you for the quick reply. We are using TPP v6.0.0-rc14 Noctilucent,
> Build 202103031119-8400 (Windows_NT-x86_64).
> As for the data let me know when you are back and I will transfer you
> either the raw file or the search results.
> Enjoy your vacation.
> Thanks,
> Oded
> On Monday, 12 April 2021 at 22:17:53 UTC+3 David Shteynberg wrote:
>
>> Hi Oded,  Which release candidate are you referring to?  The earlier
>> candidates may have a bug that is corrected in a later version. If you can
>> share some data and specifics about the missing PSMs I can run it here and
>> troubleshoot the problem.
>>
>> Thanks!
>>
>> David
>>
>> P.S. I am on vacation this week so will troubleshoot next week.
>>
>> On Mon, Apr 12, 2021, 11:49 AM Oded <[email protected]> wrote:
>>
>>> Hi there,
>>> We recently download the TPP 6.0 RC and while using it we noticed that
>>> we obtain fewer peptides IDs than what we got for the same dataset and
>>> search output with version 5.2.
>>> Many of the missing peptides seem to have decent Expect value and MS/MS
>>> following a visual inspection.
>>> This seems that this is due to changes in PeptideProphet and are not
>>> related to the search itself that was done with Comet.
>>> Is there any additional parameter that should be included in the new
>>> version in order to restore the missing peptides?
>>> Many thanks,
>>> Oded
>>>
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