Hi David,
Thank you for the quick reply. We are using TPP v6.0.0-rc14 Noctilucent, 
Build 202103031119-8400 (Windows_NT-x86_64).
As for the data let me know when you are back and I will transfer you 
either the raw file or the search results.
Enjoy your vacation.
Thanks,
Oded
On Monday, 12 April 2021 at 22:17:53 UTC+3 David Shteynberg wrote:

> Hi Oded,  Which release candidate are you referring to?  The earlier 
> candidates may have a bug that is corrected in a later version. If you can 
> share some data and specifics about the missing PSMs I can run it here and 
> troubleshoot the problem.   
>
> Thanks!
>
> David 
>
> P.S. I am on vacation this week so will troubleshoot next week.
>
> On Mon, Apr 12, 2021, 11:49 AM Oded <[email protected]> wrote:
>
>> Hi there,
>> We recently download the TPP 6.0 RC and while using it we noticed that we 
>> obtain fewer peptides IDs than what we got for the same dataset and search 
>> output with version 5.2.
>> Many of the missing peptides seem to have decent Expect value and MS/MS 
>> following a visual inspection. 
>> This seems that this is due to changes in PeptideProphet and are not 
>> related to the search itself that was done with Comet.
>> Is there any additional parameter that should be included in the new 
>> version in order to restore the missing peptides?
>> Many thanks,
>> Oded
>>
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