On 13 Aug 2013, at 11:27, [email protected] wrote:

> 
> 
> W dniu wtorek, 13 sierpnia 2013 11:24:59 UTC+1 użytkownik Valentina Erastova 
> napisał:
> 
> On 13 Aug 2013, at 11:21, [email protected] wrote:
> 
>> I used different software ACEMD driving my protein from 300K to 500K 
>> thousands of times to explore or possible conformations...so the box 
>> dimension was changing from 9.4 to 11.4 nm. At 300K it had 9.4 nm. I used 
>> all the states corresponding to 300K only. No I will change the box in my CG 
>> iterative method to 9.4 nm as I had 12 nm no clue why... Should work fine 
>> now. Thank you!
> 
> May be some inconsistence with trajectory reading. 
> Never used AceMD.
> V
> 
> 
> You better try that :) 60 ns a day with 100 K atoms using temp annealing ar 
> one GPU  :)

Just implemented material science FFs onto gromacs, not reimplementing again 
(pain). But sounds good;)


>> 
>> W dniu wtorek, 13 sierpnia 2013 11:08:21 UTC+1 użytkownik Valentina Erastova 
>> napisał:
>> So, you started with cubic box of 95A * 3  (gromacs units are nm)? Then you 
>> T annealed, got output confout.gro (with box size at the bottom of the 
>> file), change of the box size can be taken from trajectory.
>> 
>> I am not too sure about your method, tbh, as annealing is a funny thing - it 
>> can cause system to lag behind. Personally, I wouldn't use a system that 
>> potentially can not have reached equilibrium to get reference potentials for 
>> CG.
>> 
>> .tpr is binary, i.e. you cannot read it.
>> 
>> csg_stat - I am not sure, better ask developers, but I would expect it to 
>> come from the trajectory.
>> 
>> V
>> 
>> 
>> 
>> On 13 Aug 2013, at 10:56, [email protected] wrote:
>> 
>>> Sorry, my cell dimension was 95A (I was running temperature annealing so 
>>> the box dimension changed). Where in tpr file can I see the actual cell 
>>> dimension? I mean from where csg_stat takes the cell dimension to calculate 
>>> RDFs?
>>> 
>>> Steven
>>> 
>>> W dniu wtorek, 13 sierpnia 2013 10:49:13 UTC+1 użytkownik Valentina 
>>> Erastova napisał:
>>> Hi,
>>> 
>>> I am a little confused there.
>>> 
>>> You need to have a well equilibrated system before you CG. My way is to run 
>>> NPT, but make sure that the system is equilibrated and V is also constant.
>>> 
>>> When you start CG, I would use NVT, check what is the P (normally 
>>> completely off), then do a P-correction.
>>> 
>>> Good luck, V
>>> 
>>> 
>>> On 13 Aug 2013, at 10:37, <[email protected]>
>>>  wrote:
>>> 
>>>> I think I know where is the reason... I set the wrong unit cell in my CG 
>>>> model. However, I run full atomistic in NPT and the cubic unit cell was 
>>>> changing between 94A and 114A. Which shall I set up in my CG run then 
>>>> which is in NVT? 
>>>> 
>>>> W dniu poniedziałek, 12 sierpnia 2013 17:02:36 UTC+1 użytkownik Valentina 
>>>> Erastova napisał:
>>>> Hi, have you used scaling by by 1 /4 \pi r^2for bond  and  by 1/ sin 
>>>> (\theta) for angle distribution?
>>>> 
>>>> I assume those are angles & bonds.
>>>> 
>>>> Best,
>>>> V
>>>> 
>>>> 
>>>> 
>>>> On 12 Aug 2013, at 16:39, [email protected] wrote:
>>>> 
>>>>> Dear Users,
>>>>> 
>>>>> I run 7500 iterations of the protein with 5 different bead types so 15 
>>>>> distributions. Please, see attached 4 examples - black atomistic, red 
>>>>> coarse-grained.
>>>>> 14 of them have exactly the same shapes as atomistic but have higher 
>>>>> values and they stopped converging after 6000 steps. Only one (attached) 
>>>>> perfectly converged.
>>>>> May that be the reason of the one converged so its a wrong distribution 
>>>>> so the other cannot? Or everything is ok and I just to normalize them?
>>>>> 
>>>>> Thank you,
>>>>> 
>>>>> Steven
>>>>> 
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>>>>> <Dsitributions_RDF.png>
>>>> 
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