W dniu wtorek, 13 sierpnia 2013 16:08:49 UTC+1 użytkownik Christoph 
Junghans napisał:
>
> 2013/8/13  <[email protected] <javascript:>>: 
> > 
> > 
> > W dniu wtorek, 13 sierpnia 2013 14:54:16 UTC+1 użytkownik Christoph 
> Junghans 
> > napisał: 
> >> 
> >> 2013/8/13  <[email protected]>: 
> >> > Or is there any command which will tell csg_stat to get rid of PBC so 
> >> > that 
> >> > cell dimension will not have influcence on RDFs? I mean target 
> >> > distributions 
> >> > as well as during the iterations in IBI? 
> >> VOTCA reads the box from the tpr file. One could use a gro file as a 
> >> topology (--top conf.gro), but the gro file contains less information 
> >> (e.g. charges, mass, types will be wrong.). Gro files contain a box, 
> >> too, but for some reason, which I don't remember right now, VOTCA 
> >> won't use it. 
> >> 
> >> You can use a different topology file to calculate the rdfs 
> >> (cg.inverse.gromacs.topol). We originally added this feature, so that 
> >> one can calculated rdfs with a different number of exclusions, but one 
> >> can also use that to use a different box. In your case just make the 
> >> box huge. 
> >> 
> >> Cheers, 
> >> 
> >> Christoph 
> > 
> > 
> > Thank you. But when I set up the conf.gro to the box average from 
> atomistic 
> > simulations the tpr file by grompp will be set up correctly. I tried 
> this 
> > and all profiles are converging. However, the more accurate will be RDF 
> > which does not account the box size so without pbc. Is there any command 
> in 
> > csg_stat which can calculate this like g_rdf -nopbc option? I realised 
> in 
> > VMD that when changing the cell dimension RDFs changes....which makes 
> sense. 
> > When setup without box size (dont use pbc) I got normalized 
> distributions 
> > which will be more accurate in my case. 
> No, csg_stat doesn't have such an option! (see "csg_stat --help" for 
> details). 
> However, feel free to add one ;-) 
>
> Like I said before, the easiest in your case will be to create a 
> special tpr with no box beforehand and give it to csg_stat via 
> the cg.inverse.gromacs.topol option, so that the special tpr is only 
> used for the rdf calculation, but not for the simulation. 
>
> Christoph 
>

Thank you. I guess grompp will complain about no box dimension but it can 
be done I guess with maxwarining option. 
other would be to use g_rdf script you provided me, yep?

Steven 

> > 
> > Steven 
> > 
> >> 
> >> > 
> >> > Steven 
> >> > 
> >> > W dniu wtorek, 13 sierpnia 2013 11:34:51 UTC+1 użytkownik Valentina 
> >> > Erastova 
> >> > napisał: 
> >> >> 
> >> >> 
> >> >> On 13 Aug 2013, at 11:27, [email protected] wrote: 
> >> >> 
> >> >> 
> >> >> 
> >> >> W dniu wtorek, 13 sierpnia 2013 11:24:59 UTC+1 użytkownik Valentina 
> >> >> Erastova napisał: 
> >> >>> 
> >> >>> 
> >> >>> On 13 Aug 2013, at 11:21, [email protected] wrote: 
> >> >>> 
> >> >>> I used different software ACEMD driving my protein from 300K to 
> 500K 
> >> >>> thousands of times to explore or possible conformations...so the 
> box 
> >> >>> dimension was changing from 9.4 to 11.4 nm. At 300K it had 9.4 nm. 
> I 
> >> >>> used 
> >> >>> all the states corresponding to 300K only. No I will change the box 
> in 
> >> >>> my CG 
> >> >>> iterative method to 9.4 nm as I had 12 nm no clue why... Should 
> work 
> >> >>> fine 
> >> >>> now. Thank you! 
> >> >>> 
> >> >>> 
> >> >>> May be some inconsistence with trajectory reading. 
> >> >>> Never used AceMD. 
> >> >>> V 
> >> >>> 
> >> >> 
> >> >> You better try that :) 60 ns a day with 100 K atoms using temp 
> >> >> annealing 
> >> >> ar one GPU  :) 
> >> >> 
> >> >> 
> >> >> Just implemented material science FFs onto gromacs, not 
> reimplementing 
> >> >> again (pain). But sounds good;) 
> >> >> 
> >> >> 
> >> >>> 
> >> >>> W dniu wtorek, 13 sierpnia 2013 11:08:21 UTC+1 użytkownik Valentina 
> >> >>> Erastova napisał: 
> >> >>>> 
> >> >>>> So, you started with cubic box of 95A * 3  (gromacs units are nm)? 
> >> >>>> Then 
> >> >>>> you T annealed, got output confout.gro (with box size at the 
> bottom 
> >> >>>> of the 
> >> >>>> file), change of the box size can be taken from trajectory. 
> >> >>>> 
> >> >>>> I am not too sure about your method, tbh, as annealing is a funny 
> >> >>>> thing 
> >> >>>> - it can cause system to lag behind. Personally, I wouldn't use a 
> >> >>>> system 
> >> >>>> that potentially can not have reached equilibrium to get reference 
> >> >>>> potentials for CG. 
> >> >>>> 
> >> >>>> .tpr is binary, i.e. you cannot read it. 
> >> >>>> 
> >> >>>> csg_stat - I am not sure, better ask developers, but I would 
> expect 
> >> >>>> it 
> >> >>>> to come from the trajectory. 
> >> >>>> 
> >> >>>> V 
> >> >>>> 
> >> >>>> 
> >> >>>> 
> >> >>>> On 13 Aug 2013, at 10:56, [email protected] wrote: 
> >> >>>> 
> >> >>>> Sorry, my cell dimension was 95A (I was running temperature 
> annealing 
> >> >>>> so 
> >> >>>> the box dimension changed). Where in tpr file can I see the actual 
> >> >>>> cell 
> >> >>>> dimension? I mean from where csg_stat takes the cell dimension to 
> >> >>>> calculate 
> >> >>>> RDFs? 
> >> >>>> 
> >> >>>> Steven 
> >> >>>> 
> >> >>>> W dniu wtorek, 13 sierpnia 2013 10:49:13 UTC+1 użytkownik 
> Valentina 
> >> >>>> Erastova napisał: 
> >> >>>>> 
> >> >>>>> Hi, 
> >> >>>>> 
> >> >>>>> I am a little confused there. 
> >> >>>>> 
> >> >>>>> You need to have a well equilibrated system before you CG. My way 
> is 
> >> >>>>> to 
> >> >>>>> run NPT, but make sure that the system is equilibrated and V is 
> also 
> >> >>>>> constant. 
> >> >>>>> 
> >> >>>>> When you start CG, I would use NVT, check what is the P (normally 
> >> >>>>> completely off), then do a P-correction. 
> >> >>>>> 
> >> >>>>> Good luck, V 
> >> >>>>> 
> >> >>>>> 
> >> >>>>> On 13 Aug 2013, at 10:37, <[email protected]> 
> >> >>>>>  wrote: 
> >> >>>>> 
> >> >>>>> I think I know where is the reason... I set the wrong unit cell 
> in 
> >> >>>>> my 
> >> >>>>> CG model. However, I run full atomistic in NPT and the cubic unit 
> >> >>>>> cell was 
> >> >>>>> changing between 94A and 114A. Which shall I set up in my CG run 
> >> >>>>> then which 
> >> >>>>> is in NVT? 
> >> >>>>> 
> >> >>>>> W dniu poniedziałek, 12 sierpnia 2013 17:02:36 UTC+1 użytkownik 
> >> >>>>> Valentina Erastova napisał: 
> >> >>>>>> 
> >> >>>>>> Hi, have you used scaling by by 1 /4 \pi r^2for bond  and  by 1/ 
> >> >>>>>> sin 
> >> >>>>>> (\theta) for angle distribution? 
> >> >>>>>> 
> >> >>>>>> I assume those are angles & bonds. 
> >> >>>>>> 
> >> >>>>>> Best, 
> >> >>>>>> V 
> >> >>>>>> 
> >> >>>>>> 
> >> >>>>>> 
> >> >>>>>> On 12 Aug 2013, at 16:39, [email protected] wrote: 
> >> >>>>>> 
> >> >>>>>> Dear Users, 
> >> >>>>>> 
> >> >>>>>> I run 7500 iterations of the protein with 5 different bead types 
> so 
> >> >>>>>> 15 
> >> >>>>>> distributions. Please, see attached 4 examples - black 
> atomistic, 
> >> >>>>>> red 
> >> >>>>>> coarse-grained. 
> >> >>>>>> 14 of them have exactly the same shapes as atomistic but have 
> >> >>>>>> higher 
> >> >>>>>> values and they stopped converging after 6000 steps. Only one 
> >> >>>>>> (attached) 
> >> >>>>>> perfectly converged. 
> >> >>>>>> May that be the reason of the one converged so its a wrong 
> >> >>>>>> distribution so the other cannot? Or everything is ok and I just 
> to 
> >> >>>>>> normalize them? 
> >> >>>>>> 
> >> >>>>>> Thank you, 
> >> >>>>>> 
> >> >>>>>> Steven 
> >> >>>>>> 
> >> >>>>>> -- 
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> >> >>>>>> 
> >> >>>>>> 
> >> >>>>>> <Dsitributions_RDF.png> 
> >> >>>>>> 
> >> >>>>>> 
> >> >>>>> 
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> >> -- 
> >> Christoph Junghans 
> >> Web: http://www.compphys.de 
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> -- 
> Christoph Junghans 
> Web: http://www.compphys.de 
>

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