W dniu wtorek, 13 sierpnia 2013 14:54:16 UTC+1 użytkownik Christoph 
Junghans napisał:
>
> 2013/8/13  <[email protected] <javascript:>>: 
> > Or is there any command which will tell csg_stat to get rid of PBC so 
> that 
> > cell dimension will not have influcence on RDFs? I mean target 
> distributions 
> > as well as during the iterations in IBI? 
> VOTCA reads the box from the tpr file. One could use a gro file as a 
> topology (--top conf.gro), but the gro file contains less information 
> (e.g. charges, mass, types will be wrong.). Gro files contain a box, 
> too, but for some reason, which I don't remember right now, VOTCA 
> won't use it. 
>
> You can use a different topology file to calculate the rdfs 
> (cg.inverse.gromacs.topol). We originally added this feature, so that 
> one can calculated rdfs with a different number of exclusions, but one 
> can also use that to use a different box. In your case just make the 
> box huge. 
>
> Cheers, 
>
> Christoph 
>

Thank you. But when I set up the conf.gro to the box average from atomistic 
simulations the tpr file by grompp will be set up correctly. I tried this 
and all profiles are converging. However, the more accurate will be RDF 
which does not account the box size so without pbc. Is there any command in 
csg_stat which can calculate this like g_rdf -nopbc option? I realised in 
VMD that when changing the cell dimension RDFs changes....which makes 
sense. When setup without box size (dont use pbc) I got normalized 
distributions which will be more accurate in my case.

Steven
 

> > 
> > Steven 
> > 
> > W dniu wtorek, 13 sierpnia 2013 11:34:51 UTC+1 użytkownik Valentina 
> Erastova 
> > napisał: 
> >> 
> >> 
> >> On 13 Aug 2013, at 11:27, [email protected] wrote: 
> >> 
> >> 
> >> 
> >> W dniu wtorek, 13 sierpnia 2013 11:24:59 UTC+1 użytkownik Valentina 
> >> Erastova napisał: 
> >>> 
> >>> 
> >>> On 13 Aug 2013, at 11:21, [email protected] wrote: 
> >>> 
> >>> I used different software ACEMD driving my protein from 300K to 500K 
> >>> thousands of times to explore or possible conformations...so the box 
> >>> dimension was changing from 9.4 to 11.4 nm. At 300K it had 9.4 nm. I 
> used 
> >>> all the states corresponding to 300K only. No I will change the box in 
> my CG 
> >>> iterative method to 9.4 nm as I had 12 nm no clue why... Should work 
> fine 
> >>> now. Thank you! 
> >>> 
> >>> 
> >>> May be some inconsistence with trajectory reading. 
> >>> Never used AceMD. 
> >>> V 
> >>> 
> >> 
> >> You better try that :) 60 ns a day with 100 K atoms using temp 
> annealing 
> >> ar one GPU  :) 
> >> 
> >> 
> >> Just implemented material science FFs onto gromacs, not reimplementing 
> >> again (pain). But sounds good;) 
> >> 
> >> 
> >>> 
> >>> W dniu wtorek, 13 sierpnia 2013 11:08:21 UTC+1 użytkownik Valentina 
> >>> Erastova napisał: 
> >>>> 
> >>>> So, you started with cubic box of 95A * 3  (gromacs units are nm)? 
> Then 
> >>>> you T annealed, got output confout.gro (with box size at the bottom 
> of the 
> >>>> file), change of the box size can be taken from trajectory. 
> >>>> 
> >>>> I am not too sure about your method, tbh, as annealing is a funny 
> thing 
> >>>> - it can cause system to lag behind. Personally, I wouldn't use a 
> system 
> >>>> that potentially can not have reached equilibrium to get reference 
> >>>> potentials for CG. 
> >>>> 
> >>>> .tpr is binary, i.e. you cannot read it. 
> >>>> 
> >>>> csg_stat - I am not sure, better ask developers, but I would expect 
> it 
> >>>> to come from the trajectory. 
> >>>> 
> >>>> V 
> >>>> 
> >>>> 
> >>>> 
> >>>> On 13 Aug 2013, at 10:56, [email protected] wrote: 
> >>>> 
> >>>> Sorry, my cell dimension was 95A (I was running temperature annealing 
> so 
> >>>> the box dimension changed). Where in tpr file can I see the actual 
> cell 
> >>>> dimension? I mean from where csg_stat takes the cell dimension to 
> calculate 
> >>>> RDFs? 
> >>>> 
> >>>> Steven 
> >>>> 
> >>>> W dniu wtorek, 13 sierpnia 2013 10:49:13 UTC+1 użytkownik Valentina 
> >>>> Erastova napisał: 
> >>>>> 
> >>>>> Hi, 
> >>>>> 
> >>>>> I am a little confused there. 
> >>>>> 
> >>>>> You need to have a well equilibrated system before you CG. My way is 
> to 
> >>>>> run NPT, but make sure that the system is equilibrated and V is also 
> >>>>> constant. 
> >>>>> 
> >>>>> When you start CG, I would use NVT, check what is the P (normally 
> >>>>> completely off), then do a P-correction. 
> >>>>> 
> >>>>> Good luck, V 
> >>>>> 
> >>>>> 
> >>>>> On 13 Aug 2013, at 10:37, <[email protected]> 
> >>>>>  wrote: 
> >>>>> 
> >>>>> I think I know where is the reason... I set the wrong unit cell in 
> my 
> >>>>> CG model. However, I run full atomistic in NPT and the cubic unit 
> cell was 
> >>>>> changing between 94A and 114A. Which shall I set up in my CG run 
> then which 
> >>>>> is in NVT? 
> >>>>> 
> >>>>> W dniu poniedziałek, 12 sierpnia 2013 17:02:36 UTC+1 użytkownik 
> >>>>> Valentina Erastova napisał: 
> >>>>>> 
> >>>>>> Hi, have you used scaling by by 1 /4 \pi r^2for bond  and  by 1/ 
> sin 
> >>>>>> (\theta) for angle distribution? 
> >>>>>> 
> >>>>>> I assume those are angles & bonds. 
> >>>>>> 
> >>>>>> Best, 
> >>>>>> V 
> >>>>>> 
> >>>>>> 
> >>>>>> 
> >>>>>> On 12 Aug 2013, at 16:39, [email protected] wrote: 
> >>>>>> 
> >>>>>> Dear Users, 
> >>>>>> 
> >>>>>> I run 7500 iterations of the protein with 5 different bead types so 
> 15 
> >>>>>> distributions. Please, see attached 4 examples - black atomistic, 
> red 
> >>>>>> coarse-grained. 
> >>>>>> 14 of them have exactly the same shapes as atomistic but have 
> higher 
> >>>>>> values and they stopped converging after 6000 steps. Only one 
> (attached) 
> >>>>>> perfectly converged. 
> >>>>>> May that be the reason of the one converged so its a wrong 
> >>>>>> distribution so the other cannot? Or everything is ok and I just to 
> >>>>>> normalize them? 
> >>>>>> 
> >>>>>> Thank you, 
> >>>>>> 
> >>>>>> Steven 
> >>>>>> 
> >>>>>> -- 
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> >>>>>> 
> >>>>>> 
> >>>>>> <Dsitributions_RDF.png> 
> >>>>>> 
> >>>>>> 
> >>>>> 
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>
>
>
> -- 
> Christoph Junghans 
> Web: http://www.compphys.de 
>

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