pH is the culprit here Like some already mentioned change your pH to 6.4 and use a different buffer like cacodylate or you can use Zinc acetate in water pH still would be 6.4
from your last mail why would you add Zn to your initial hit condition is there any rationale? Padayatti On Fri, May 11, 2012 at 12:26 PM, Rajesh Kumar <ccp4...@hotmail.com> wrote: > Dear All, > > This question sounds simple but I dont know the answer. > I was preparing a 24 well crystal screen. When I try to use 10 mM ZnSO4 > with HEPES (pH 7.6) buffer it precipitates. I tried both ZnCl2 and Zn > acetate the effect is same. > I dont know why this Zn in not compatible with HEPES. > Could you please tell me why is this? > I appreciate your help. > > Thanks > Rajesh -- Pius S Padayatti,PhD, Phone: 216-658-4528
