Just to make sure I understand pH correctly: isn't it true that the [OH-] should always be the same at a given pH (by definition)?
JPK On Fri, May 11, 2012 at 11:48 AM, Katherine Sippel < katherine.sip...@gmail.com> wrote: > That is probably because you pH Tris with HCl rather than HEPES with NaOH. > The Ksp for Zn(OH)2 is 3x10^17 so the excess hydroxides are probably what > are killing your solution. > > Cheers, > > Katherine > > > On Fri, May 11, 2012 at 11:43 AM, Rajesh Kumar <ccp4...@hotmail.com>wrote: > >> If there is no cure , then fine. >> pH may not be the answer as it doesn't Happen with TRIS buffer pH 7.6. >> Thanks to every one >> Rajesh >> >> >> ------------------------------ >> Date: Fri, 11 May 2012 12:35:45 -0400 >> From: dj...@cornell.edu >> >> Subject: Re: [ccp4bb] zinc with HEPES >> To: CCP4BB@JISCMAIL.AC.UK >> >> >> >> There is no cure for HEPES. >> >> >> >> >> -- >> ======================================================================= >> All Things Serve the Beam >> ======================================================================= >> David J. Schuller >> modern man in a post-modern world >> MacCHESS, Cornell University >> schul...@cornell.edu >> >> > -- ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program email: j-kell...@northwestern.edu *******************************************
