Dear Powell, Isn't it there a way to data mine the PDB or the other repository source for the time/duration/days of the crystals obtained.
Dr. Jayashankar Selvadurai Hannover Germany On Mon, Feb 4, 2013 at 5:10 PM, Harry Powell <ha...@mrc-lmb.cam.ac.uk>wrote: > Hi David > > try going back to the one that started it all,* myoglobin, a recipe is at > > http://www.rigaku.com/products/protein/recipes > > (* feel free to argue about this) > > On 4 Feb 2013, at Mon4 Feb 16:03, David Roberts wrote: > > So, I know I say this every time I post on this board, but here it goes > again. > > I'm at an undergrad only school, and every 2 years I teach a class in > protein crystallography. This year I'm being super ambitious, and I'm > going to take a class of 16 to the synchrotron for data collection. It's > just an 8 hour thing, to show them the entire process. I'm hoping that > we can collect 5-6 good data sets while there. > > I would like them to grow their own crystals, and go collect data. Then > we'd come back and actually do a molecular replacement (pretty > easy/standard really). Just to get a feel for how it works. > > The protein I do research on is not one that I would push on this, as the > crystals are hard to grow, they are very soft, and the data just isn't the > best (resolution issues). I do have a few that will work on my proteins, > but I was thinking of having others in the class grow up classic proteins > for data collection. Obviously lysozyme is one, but I was wondering what > other standard bulletproof conditions are out there. > > Can you all suggest some protein crystallization conditions (along with > cryo conditions) for some commercially available proteins? I'm looking > to get 6-8 different ones (and we'll just take them and see how it goes). > I wouldn't mind knowing unit cell parameters as well (just a citation > works, I can have them figure it out). I have about 7 weeks to get > everything grown and frozen and ready to go. > > Any help would be greatly appreciated. It always amazes me how helpful > this group is. Thank you very much. > > Dave > > > Harry > -- > Dr Harry Powell, MRC Laboratory of Molecular Biology, MRC Centre, Hills > Road, Cambridge, CB2 0QH > Chairman of European Crystallographic Association SIG9 (Crystallographic > Computing) > > > > >
