I don't know. Is there?
On 4 Feb 2013, at Mon4 Feb 16:15, Jayashankar wrote:
Dear Powell,
Isn't it there a way to data mine the PDB or the other repository
source for the time/duration/days of the crystals obtained.
Dr. Jayashankar Selvadurai
Hannover
Germany
On Mon, Feb 4, 2013 at 5:10 PM, Harry Powell <harry@mrc-
lmb.cam.ac.uk> wrote:
Hi David
try going back to the one that started it all,* myoglobin, a recipe
is at
http://www.rigaku.com/products/protein/recipes
(* feel free to argue about this)
On 4 Feb 2013, at Mon4 Feb 16:03, David Roberts wrote:
So, I know I say this every time I post on this board, but here it
goes again.
I'm at an undergrad only school, and every 2 years I teach a class
in protein crystallography. This year I'm being super ambitious,
and I'm going to take a class of 16 to the synchrotron for data
collection. It's just an 8 hour thing, to show them the entire
process. I'm hoping that we can collect 5-6 good data sets while
there.
I would like them to grow their own crystals, and go collect data.
Then we'd come back and actually do a molecular replacement
(pretty easy/standard really). Just to get a feel for how it works.
The protein I do research on is not one that I would push on this,
as the crystals are hard to grow, they are very soft, and the data
just isn't the best (resolution issues). I do have a few that
will work on my proteins, but I was thinking of having others in
the class grow up classic proteins for data collection. Obviously
lysozyme is one, but I was wondering what other standard
bulletproof conditions are out there.
Can you all suggest some protein crystallization conditions (along
with cryo conditions) for some commercially available proteins?
I'm looking to get 6-8 different ones (and we'll just take them
and see how it goes). I wouldn't mind knowing unit cell
parameters as well (just a citation works, I can have them figure
it out). I have about 7 weeks to get everything grown and frozen
and ready to go.
Any help would be greatly appreciated. It always amazes me how
helpful this group is. Thank you very much.
Dave
Harry
--
Dr Harry Powell, MRC Laboratory of Molecular Biology, MRC Centre,
Hills Road, Cambridge, CB2 0QH
Chairman of European Crystallographic Association SIG9
(Crystallographic Computing)
Harry
--
Dr Harry Powell, MRC Laboratory of Molecular Biology, MRC Centre,
Hills Road, Cambridge, CB2 0QH
Chairman of European Crystallographic Association SIG9
(Crystallographic Computing)
