Along these lines, what reagents do people use to promote disuflide bonds,
i.e., the "anti-DTT?"

Glutathione (red) + Glutathione (ox), redox potential is adjusted by varying the ratio.

Best,
Clemens



JPK

On Thu, Feb 28, 2013 at 2:06 AM, David Briggs <drdavidcbri...@gmail.com>wrote:

You might want to try "Disulfide by design"

http://cptweb.cpt.wayne.edu/DbD2/

Cheers

Dave
On Feb 28, 2013 6:55 AM, "Careina Edgooms" <careinaedgo...@yahoo.com>
wrote:

Dear CCP4 members

I wish to engineer a disulfide bond at the dimer interface of a protein I
am working with. Does anyone know of any available software to assist with
this?

Best
Careina




--
*******************************************
Jacob Pearson Keller, PhD
Postdoctoral Associate
HHMI Janelia Farms Research Campus
email: j-kell...@northwestern.edu
*******************************************




--------------------------------------------------
Dr. Clemens Grimm
Institut für Biochemie
Biozentrum der Universität Würzburg
Am Hubland
D-97074 Würzburg
Germany
e-mail: clemens.gr...@biozentrum.uni-wuerzburg.de
phone : +49 0931 31 84031
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