I don't know how much mileage you'd get out of it with your protein, but
I was able to get very efficient disulfide linkage at the dimerization
interface of my protein by dropping the salt to nearly nothing and
running lots of buffer over it after immobilization on a cation exchange
column (during purification).
--p
On 02/28/2013 06:09 AM, Jacob Keller wrote:
Along these lines, what reagents do people use to promote disuflide
bonds, i.e., the "anti-DTT?"
JPK
On Thu, Feb 28, 2013 at 2:06 AM, David Briggs
<drdavidcbri...@gmail.com <mailto:drdavidcbri...@gmail.com>> wrote:
You might want to try "Disulfide by design"
http://cptweb.cpt.wayne.edu/DbD2/
Cheers
Dave
On Feb 28, 2013 6:55 AM, "Careina Edgooms"
<careinaedgo...@yahoo.com <mailto:careinaedgo...@yahoo.com>> wrote:
Dear CCP4 members
I wish to engineer a disulfide bond at the dimer interface of
a protein I am working with. Does anyone know of any available
software to assist with this?
Best
Careina
--
*******************************************
Jacob Pearson Keller, PhD
Postdoctoral Associate
HHMI Janelia Farms Research Campus
email: j-kell...@northwestern.edu <mailto:j-kell...@northwestern.edu>
*******************************************
