[ccp4bb] NYU - cryo EM staff scientist positions

[Gira Bhabha]

Hi All, Please see below for two staff scientist positions available at our 
Cryo EM core facility at NYU. Feel free to email me directly with questions! 
Thanks, Gira


———
Cryo EM Staff Scientist positions at NYU



Location: Manhattan, New York

To apply: Please send a cover letter, CV and names of 3 references to: 
adriana.he...@nyulangone.org 

The NYU Cryo-EM Core is looking for staff scientists. The facility is a 
state-of-the-art shared resource for biomedical researchers. The Cryo-EM staff 
members develop and provide their knowledge and expertise to the research 
community at NYU Langone in applying cryo-EM imaging to their research. 
Specific responsibilities include operation of the existing two cryo-electron 
microscopes and ancillary equipment for sample preparation, as well as 
maintaining accurate logs/records for microscope performance, usage and 
service, as well development and updating of Standard Operating Procedures. The 
successful candidate will be expected to learn new procedures as they are 
developed and implemented by the core. Good communication skills and an open, 
collaborative attitude are a must. This facility will be optimized for high 
throughput data collection, thus fitting into a pipeline that maximizes the 
efficiency and productivity will be a key part of this job. The NYU research 
community includes inexperienced groups with potential cryo-EM projects. This 
job requires the implementation of training plans and providing support and 
advice to inexperienced users during the initial phase of their projects. 



Preferred Qualifications:

M.S in structural biology, biochemistry or related field, Ph.D. would be a plus.
2-3 years of experience in EM including handling samples, using microscopes and 
evaluation of images; cryo EM experience preferred but not required; experience 
with Arctica or Krios would be bonus 
Knowledge of the principles of transmission electron microscopy and 3D 
structure determination 
Good communication and interpersonal skills 
Ability to multitask and learn complex new procedures
———


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Re: [ccp4bb] clashscore question

[Xiaodi Yu]

Thank you all for the input. I think my problem got solved.

The structure was determined by cryo-EM at 3.2 A. I used 
phenix.real_space_refine to refine the structure using the model with no 
hydrogen. the statistic report directly from phenix was pretty good but not 
from pdb validation server (especially the clashscore changes a lot). As John 
and Sharan mentioned here that the PDB server calculates the clashscore with 
the hydrogen added and that makes the difference.

What I did is adding the hydrogen first and followed by one run of phenix 
refinement. Finally, the problem got solved, the clashscore was dropped to 2 
reported from both the phenix and PDB server. I do consider the alternative 
conformation and symmetry. While I think adding the hydrogen is the key to my 
case.

Thank you again for your kindly suggestion. I wish my case may serve the people 
who have the similar problem in the future.

Xiaodi


From: CCP4 bulletin board  on behalf of John Berrisford 

Sent: Wednesday, January 16, 2019 12:06 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] clashscore question


Dear Xiaodi



The wwPDB validation service does indeed add hydrogens using Reduce before 
calculating clashes.

For more details of what processes are run when generating the wwPDB validation 
report see the on-line help

https://www.wwpdb.org/validation/2017/XrayValidationReportHelp#close_contacts



If you would like us to look into this in more detail please can you email us 
directly on

validat...@mail.wwpdb.org

with details of your validation session.



This email address is also listed on the wwPDB validation help page

https://www.wwpdb.org/validation/validation-reports



Regards



John



From: CCP4 bulletin board  On Behalf Of Sharan Karade
Sent: 16 January 2019 04:28
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] clashscore question



Dear Yu,



I think pdb validation server add hydrogen to the structure and then calculate 
the clashscore. The other methods you mentioned calculate clashscore without 
adding hydrogen to the structure.



Regards

Sharan



On Tue, Jan 15, 2019, 11:23 PM Xiaodi Yu 
mailto:uppsala@hotmail.com> wrote:

Hi All:



I have a pdb file and the clashscores reported from both phenix and MolProbity 
were around 5, while the clashscore from the pdb validation server is 17. I 
wonder what may cause the difference? Any suggestion is appreciated.



Thank you.



Xiaodi





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Re: [ccp4bb] hybrid photon counter in the home lab

[Tim Gruene]

Dear Wolfram,

the principles of data integration do not differ much between small molecule and
macromolecular data (XDS, DIALS, HKL2000, mosflm, and SAINT can be used for
either), hence the advantages reported for chemical crystallography should apply
likewise for macromolecular crystals.

In addition to what is listed for small molecule, the problem of overlapping
reflections due to large unit cell axis might slighlty improve with hybrid pixel
detectors, when the point spread is less than for CCD et al.

Best,
Tim


On Tue, Jan 15, 2019 at 01:20:06PM -0500, wtempel wrote:
> Hi,
> I would value your opinions in this equipment-related question.
> Allé et al have compared detector types with a molybdemon source for a
> small molecule application
> . Are there similar
> published comparisons for protein crystallography? What benefits can I
> expect from replacing a CCD detector with a hybrid photon counter at an
> energy of 8 keV and in the absence of the flux that a modern synchrotron
> provides?
> 
> Thank you in advance.
> Wolfram Tempel
> 
> 
> 
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

-- 
--
Tim Gruene
GPG Key ID = A46BEE1A




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signature.asc
Description: PGP signature

Re: [ccp4bb] clashscore question

[John Berrisford]

Dear Xiaodi 

 

The wwPDB validation service does indeed add hydrogens using Reduce before 
calculating clashes. 

For more details of what processes are run when generating the wwPDB validation 
report see the on-line help

https://www.wwpdb.org/validation/2017/XrayValidationReportHelp#close_contacts

 

If you would like us to look into this in more detail please can you email us 
directly on 

validat...@mail.wwpdb.org  

with details of your validation session.

 

This email address is also listed on the wwPDB validation help page

https://www.wwpdb.org/validation/validation-reports

 

Regards

 

John

 

From: CCP4 bulletin board  On Behalf Of Sharan Karade
Sent: 16 January 2019 04:28
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] clashscore question

 

Dear Yu,

 

I think pdb validation server add hydrogen to the structure and then calculate 
the clashscore. The other methods you mentioned calculate clashscore without 
adding hydrogen to the structure.

 

Regards

Sharan

 

On Tue, Jan 15, 2019, 11:23 PM Xiaodi Yu mailto:uppsala@hotmail.com>  wrote:

Hi All:

 

I have a pdb file and the clashscores reported from both phenix and MolProbity 
were around 5, while the clashscore from the pdb validation server is 17. I 
wonder what may cause the difference? Any suggestion is appreciated.

 

Thank you.

 

Xiaodi 

 

  _  

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[ccp4bb] ReNaFoBiS. Oleron 2019. Registration is now open

[VIE]

ReNaFoBiS. Oleron 2019. Registration is now open

We are pleased to announce the 6th RéNaFoBiS Oléron workshop, dedicated to 
Integrative Structural Biology, that will take place on the Oléron island 
(France), from June the 21th until June the 28th, 2019. The main objective of 
this workshop is to offer a theoretical and practical training in the different 
techniques used in integrative structural biology (X-ray diffraction, small 
angle X-ray scattering, NMR,cryo-electron microscopy, sample preparation for 
structural biology,biophysical methods to study and characterize macromolecular 
interactions). The goals are to explain and illustrate, to an audience mainly 
composed of doctoral students and young researchers, the contributions and 
limitations of each method with a strong emphasis on their complementarity and 
future developments.

The official language of the workshop is French but presentations may be given 
in English, depending on the overall profile of the students. For
practicals, English and French speaking groups may be organised.

Registration is now open on https://ecolebios2019.sciencesconf.org/

Applications will be evaluated upon deposition.

Best regards
Jean Cavarelli
National ReNaFoBiS Coordinator
https://www.renafobis.fr/
--

Jean Cavarelli
Professor of Structural Biology
Tél : +33 (0)3 69 48 52 74
jean.cavare...@unistra.fr
Structural Biology of Epigenetic Targets
Integrated Structural Biology Department
IGBMC. UMR7104 CNRS-UDS, INSERM U964.
F - 67404 Illkirch



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[ccp4bb] PhD studentship at University of Geneva, Switzerland; Structural and biochemical analysis of inositol pyrophosphate signaling pathways

[Michael Hothorn]

Applications are invited for a PhD position, starting any time in 2019, 
in the group Prof. Michael Hothorn at University of Geneva 
(http://structplantbio.org). The lab combines structural biology (X-ray 
protein crystallography, cryo electron microscopy) and quantitative 
biochemistry with genetics and cell biology to dissect novel signal 
transduction pathways required for plant growth and development. The 
successful candidate will investigate how inositol pyrophosphate 
signaling molecules involved in nutrient signaling interact with 
different plant signaling proteins and protein complexes. She/he will 
use state-of-the art molecular biology, protein biochemistry and 
structural biology techniques to characterize these interactions in 
molecular detail.


The candidate should have a first class degree in biochemistry or 
closely related subject and ideally prior experience in molecular 
biology and in protein biochemistry. A basic knowledge of protein 
expression and purification, quantitative biochemistry, and in protein 
crystallography or cryoEM would be highly appreciated.


Applications should be sent Prof. Michael Hothorn 
(michael.hoth...@unige.ch) as a single PDF document, containing a 2 page 
CV (including details on the final grade or the expected grades) and a 
motivational statement of up to 2 pages. Applicants should also include 
contact details of 2-3 academic referees.


Informal inquiries can be addressed by email to Prof. Michael Hothorn  
(michael.hoth...@unige.ch). More information can be found here: 
http://structplantbio.org


The successful candidate will receive an internally funded, full-time 
PhD contract for up to 5 years.


_References_

Zhu J, Lau K, Harmel RK, Puschmann R, Broger L, Dutta AK, Jessen HJ, 
Hothorn LA, Fiedler D, Hothorn M (2019) Two bifunctional inositol 
pyrophosphate kinases/phosphatases control plant phosphate homeostasis. 
bioRxiv doi: https://doi.org/10.1101/467076
Hohmann U, Nicolet J, Moretti A, Hothorn LA, Hothorn M (2018) The SERK3 
elongated allele defines a role for BIR ectodomains in brassinosteroid 
signalling. Nature Plants 4:345-351 doi: 10.1038/s41477-018-0150-9
Hohmann U, Santiago J, Nicolet J, Olsson V, Spiga FM, Hothorn LA, 
Butenko MA, Hothorn M (2018) Mechanistic basis for the activation of 
plant membrane receptor kinases by SERK-family coreceptors. PNAS 
115(13):3488-3493
Wild R†, Gerasimaite R†, Jung JY†, Truffault V, Pavlovic I, Schmidt A, 
Saiardi A, Jessen HJ, Poirier Y*, Hothorn M*, Mayer A* (2016) Control of 
eukaryotic phosphate homeostasis by inositol polyphosphate sensor 
domains. Science 352(6288):986-90





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[ccp4bb] HFIR/SNS Advanced Neutron Diffraction and Scattering Workshop - HANDS 2019

[Meilleur, Flora]

First announcement

HANDS 2019
HFIR/SNS Advanced Neutron Diffraction and Scattering Workshop
Oak Ridge, TN. June 19 - June 14, 2018

Oak Ridge National Laboratory, in conjunction with North Carolina State 
University, and support from the National Science Foundation and the Shull 
Wollan Center - a Joint Institute for Neutron Scattering, celebrates a decade 
of neutron scattering education in structural biology at ORNL with the HFIR/SNS 
Advanced Neutron Diffraction and Scattering workshop (HANDS 2019).

Detailed information can be found on the workshop web page: 
https://conference.sns.gov/hands2019/
Application deadline: April 29, 2019
Fellowships to support travel and accommodation are available. No registration 
fee.

The workshop aims at enabling structural biologists to fully exploit the latest 
instrumentation and software development at the SNS and HFIR facilities at Oak 
Ridge National Laboratory. Participants of HANDS 2019 will become familiar with 
neutron techniques with hands-on experiments in sample preparation, 
crystallography, small angle scattering, reflectometry and neutron spin echo. 
The workshop is designed for graduate students, post-doctoral fellows and 
faculty with limited to no experience with neutron sciences.

Flora Meilleur, Ph. D
Neutron Scattering Scientist, Neutron Scattering Division
Oak Ridge National Laboratory
Associate Professor, Molecular and Structural Biochemistry
North Carolina State University




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Re: [ccp4bb] hybrid photon counter in the home lab

[Keller, Jacob]

Wow, that’s pretty impressive. I hadn’t realized the high levels of DQE you had 
achieved. If you could make one for visible photons, you’d make a lot of 
microscopists really happy.

I also had not realized that QE of other detectors is intensity dependent—seems 
counter-intuitive.

JPK

+
Jacob Pearson Keller
Research Scientist / Looger Lab
HHMI Janelia Research Campus
19700 Helix Dr, Ashburn, VA 20147
Desk: (571)209-4000 x3159
Cell: (301)592-7004
+

The content of this email is confidential and intended for the recipient 
specified in message only. It is strictly forbidden to share any part of this 
message with any third party, without a written consent of the sender. If you 
received this message by mistake, please reply to this message and follow with 
its deletion, so that we can ensure such a mistake does not occur in the future.

From: Marcus Winter 
Sent: Wednesday, January 16, 2019 11:25 AM
To: Keller, Jacob ; CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] hybrid photon counter in the home lab



Dear Jacob,


Thank you for your reply.  You’re correct, of course.  As shown below and in 
the attachment, with Si sensors and at a photon energy of the 8 keV, the DQE of 
both the HyPix-6000HE and Pilatus3 R 200K HPC detectors are both considerably 
above 90%.


[cid:image001.png@01D4AD9E.60DA3C20]


Most importantly, this high DQE is maintained throughout the operating regime: 
from the highest to the lowest count rates.  As can be seen, this is in 
contrast to the highest performing CCD detector (the Atlas S2) and CMOS type 
detectors.  The high DQE even at the lowest count rates / lowest reflection 
intensities is the reason for the best quality highest resolution data to be 
collectable using the HPC technology detectors.


Many Thanks, Yours sincerely,

Marcus Winter
Rigaku.


From: Keller, Jacob [mailto:kell...@janelia.hhmi.org]
Sent: Wednesday, January 16, 2019 2:13 PM
To: Marcus Winter mailto:marcus.win...@rigaku.com>>; 
CCP4BB@JISCMAIL.AC.UK
Subject: RE: [ccp4bb] hybrid photon counter in the home lab

Doesn’t the phrase “each-and-every single photon counting capability” imply 
that quantum efficiency is 100%? I don’t think this is possible—what is the 
quantum efficiency of these detectors?

JPK

+
Jacob Pearson Keller
Research Scientist / Looger Lab
HHMI Janelia Research Campus
19700 Helix Dr, Ashburn, VA 20147
Desk: (571)209-4000 x3159
Cell: (301)592-7004
+

The content of this email is confidential and intended for the recipient 
specified in message only. It is strictly forbidden to share any part of this 
message with any third party, without a written consent of the sender. If you 
received this message by mistake, please reply to this message and follow with 
its deletion, so that we can ensure such a mistake does not occur in the future.

From: CCP4 bulletin board mailto:CCP4BB@JISCMAIL.AC.UK>> 
On Behalf Of Marcus Winter
Sent: Wednesday, January 16, 2019 3:28 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] hybrid photon counter in the home lab



Dear Wolfram,


For the past several years, Rigaku has been installing only HPC (Hybrid Photon 
Counting) X-ray area detector -based diffractometer systems: in conjunction 
with microfocus rotating anode, microfocus sealed-tube and other X-ray sources. 
 (Most recently, we have been deploying the Rigaku HyPix-6000HE HPC detector.)  
The advantages of the HPC technology over any of the phosphor-based detector 
types (CCD, CMOS and CPAD, etc…) are notable: most particularly the discrete 
each-and-every single photon counting capability, extremely high dynamic range, 
small pixel size and the single pixel point function and very rapid read-out.  
With these advantages, significantly higher resolution data can be achieved, - 
and more speedily, compared to the other detector types.  (We have a number of 
established ‘application note’ examples.)  In order to prove the advantages for 
your purposes, the best might be to collect data from a range of your own 
crystal samples.  – There is an open invitation for any seriously interested 
groups to collect data from their crystals in our applications labs.  Please 
contact your local good Rigaku salesperson.

Many Thanks, Yours sincerely,

Marcus Winter
Rigaku.


From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of wtempel
Sent: Tuesday, January 15, 2019 6:20 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] hybrid photon counter in the home lab


Hi,
I would value your opinions in this equipment-related question.
Allé et al have compared detector types with a molybdemon source for a small 
molecule application. Are 
there similar published comparisons for protein 

[ccp4bb] PhD studentship at Queen Mary University of London (Targeting myosin for heart disease: a structure-based approach to design conformation-selective modulators)

[Arianna Fornili]

Applications are invited for a PhD studentship starting from September 2019 in 
Dr. Arianna Fornili’s group at Queen Mary University of London and in 
collaboration with Dr. Julien Ochala at King’s College London.
The aim of the project is to design and test small molecules that can either 
inhibit or activate cardiac myosin, a key component of the molecular machinery 
involved in heart contraction. A combined computational/experimental approach 
will be used, which will involve the use of molecular dynamics, molecular 
docking, virtual screening and in vitro biophysical techniques.
Applications are welcome from outstanding students with, or expecting to 
obtain, a first or upper-second class honours degree in Chemistry, 
Pharmaceutical Chemistry, Biochemistry, Physics, Biophysics or related 
disciplines. A masters degree is desirable. Previous experience in molecular 
modelling/simulation is essential, previous experience in computer programming 
and/or experimental biophysical techniques is a plus.
The studentship is funded by EPRSC and will cover tuition fees and an annual 
tax-free maintenance allowance at the Research Council rate (£16,777 in 
2018/19) for 3.5 years. UK students, and EU students who have been ordinarily 
resident in the UK for at least 3 years are normally eligible for full EPSRC 
funding.
For more information:
https://www.qmul.ac.uk/sbcs/postgraduate/phd-programmes/projects/display-title-649759-en.html
https://www.findaphd.com/phds/project/targeting-myosin-for-heart-disease-a-structure-based-approach-to-design-conformation-selective-modulators/?p105203
Before submitting a formal application online, please contact Dr. Fornili 
(a.forn...@qmul.ac.uk) and include your CV, your 
academic transcripts, a cover letter explaining eligibility and interest in the 
project and the contact details of two academic referees. The deadline for 
formal applications is on the 31st of January 2019.
Dr. Arianna Fornili
Lecturer in Computational Organic Chemistry
School of Biological and Chemical Sciences
Joseph Priestley Building (room G.04)
Queen Mary, University of London
Mile End Road
E1 4NS London UK
email: a.forn...@qmul.ac.uk
Tel: +44 (0) 207 882 8446
website: https://afornililab.wordpress.com




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Re: [ccp4bb] hybrid photon counter in the home lab

[Keller, Jacob]

Doesn’t the phrase “each-and-every single photon counting capability” imply 
that quantum efficiency is 100%? I don’t think this is possible—what is the 
quantum efficiency of these detectors?

JPK

+
Jacob Pearson Keller
Research Scientist / Looger Lab
HHMI Janelia Research Campus
19700 Helix Dr, Ashburn, VA 20147
Desk: (571)209-4000 x3159
Cell: (301)592-7004
+

The content of this email is confidential and intended for the recipient 
specified in message only. It is strictly forbidden to share any part of this 
message with any third party, without a written consent of the sender. If you 
received this message by mistake, please reply to this message and follow with 
its deletion, so that we can ensure such a mistake does not occur in the future.

From: CCP4 bulletin board  On Behalf Of Marcus Winter
Sent: Wednesday, January 16, 2019 3:28 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] hybrid photon counter in the home lab



Dear Wolfram,


For the past several years, Rigaku has been installing only HPC (Hybrid Photon 
Counting) X-ray area detector -based diffractometer systems: in conjunction 
with microfocus rotating anode, microfocus sealed-tube and other X-ray sources. 
 (Most recently, we have been deploying the Rigaku HyPix-6000HE HPC detector.)  
The advantages of the HPC technology over any of the phosphor-based detector 
types (CCD, CMOS and CPAD, etc…) are notable: most particularly the discrete 
each-and-every single photon counting capability, extremely high dynamic range, 
small pixel size and the single pixel point function and very rapid read-out.  
With these advantages, significantly higher resolution data can be achieved, - 
and more speedily, compared to the other detector types.  (We have a number of 
established ‘application note’ examples.)  In order to prove the advantages for 
your purposes, the best might be to collect data from a range of your own 
crystal samples.  – There is an open invitation for any seriously interested 
groups to collect data from their crystals in our applications labs.  Please 
contact your local good Rigaku salesperson.

Many Thanks, Yours sincerely,

Marcus Winter
Rigaku.


From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of wtempel
Sent: Tuesday, January 15, 2019 6:20 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] hybrid photon counter in the home lab


Hi,
I would value your opinions in this equipment-related question.
Allé et al have compared detector types with a molybdemon source for a small 
molecule application. Are 
there similar published comparisons for protein crystallography? What benefits 
can I expect from replacing a CCD detector with a hybrid photon counter at an 
energy of 8 keV and in the absence of the flux that a modern synchrotron 
provides?

Thank you in advance.
Wolfram Tempel



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[ccp4bb] BCA Spring Meeting 2019 in Nottingham

[Vidya Darbari]

Dear All,

The British Crystallographic Association (BCA) Spring Meeting is taking place 
this year at the University f Nottingham from Monday 15th April to Thursday 
18th April, 2019. With a range of sessions highlighting integrated approaches 
to structural biology and the latest developments in X-ray crystallographic 
techniques, this year’s BCA Spring Meeting in Nottingham has something for 
everyone.
Please take a look at http://www.bcaspringmeetings.org.uk/home for full details 
of the scientific program and registration links.

Key Dates: Abstract Deadline: Friday 15th Feb, 2019
Early Bird registration deadline: Monday, 11th March 2019
YCG meeting: 15th-16th April.

Highlights of the Biological Structures Group Talks:

BSG Plenary Talk: Prof. Liz Carpenter
BSG Sessions: Novel Data collection strategies: Keynote by Florent Cipriani 
(Chair: Ramona Duman)
Complementary Structural Biology techniques: Keynote by Dmitri Svergun (Chair: 
James Garnett)
Novel Crystallisation strategies: Keynote by Apirat Chaikaud (Chair: Claire 
Naylor)
Computational Structural Biology: Keynote by Gianni de Fabritis (Chair: Shozeb 
Haider)
Multi-technique for solving large macromolecular complexes: Keynote by Carolyn 
Moores (Chair: Christos Savva)
EU Network for Rationalising Membrane Protein crystallisation : Keynote by 
Adrian Goldman (Chair: Arwen Pearson)

Looking forward to seeing you there,

Vidya Darbari and Claire Naylor





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[ccp4bb] hybrid photon counter in the home lab

[Marcus Winter]

Dear Wolfram,


For the past several years, Rigaku has been installing only HPC (Hybrid Photon 
Counting) X-ray area detector -based diffractometer systems: in conjunction 
with microfocus rotating anode, microfocus sealed-tube and other X-ray sources. 
 (Most recently, we have been deploying the Rigaku HyPix-6000HE HPC detector.)  
The advantages of the HPC technology over any of the phosphor-based detector 
types (CCD, CMOS and CPAD, etc…) are notable: most particularly the discrete 
each-and-every single photon counting capability, extremely high dynamic range, 
small pixel size and the single pixel point function and very rapid read-out.  
With these advantages, significantly higher resolution data can be achieved, - 
and more speedily, compared to the other detector types.  (We have a number of 
established ‘application note’ examples.)  In order to prove the advantages for 
your purposes, the best might be to collect data from a range of your own 
crystal samples.  – There is an open invitation for any seriously interested 
groups to collect data from their crystals in our applications labs.  Please 
contact your local good Rigaku salesperson.

Many Thanks, Yours sincerely,

Marcus Winter
Rigaku.


From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of wtempel
Sent: Tuesday, January 15, 2019 6:20 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] hybrid photon counter in the home lab


Hi,
I would value your opinions in this equipment-related question.
Allé et al have compared detector types with a molybdemon source for a small 
molecule application. Are 
there similar published comparisons for protein crystallography? What benefits 
can I expect from replacing a CCD detector with a hybrid photon counter at an 
energy of 8 keV and in the absence of the flux that a modern synchrotron 
provides?

Thank you in advance.
Wolfram Tempel



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