Re: [ccp4bb] hybrid photon counter in the home lab

[colin.n...@diamond.ac.uk]

Jacob
For some of these detectors, it is all quite complicated. See for example paper 
by Martin Stanton et al.
https://www.sciencedirect.com/science/article/pii/0168900293904057
I am sure there are other analyses but this indicates the complexity
Cheers
   Colin

From: CCP4 bulletin board  On Behalf Of Keller, Jacob
Sent: 16 January 2019 18:21
To: ccp4bb 
Subject: Re: [ccp4bb] hybrid photon counter in the home lab

Wow, that’s pretty impressive. I hadn’t realized the high levels of DQE you had 
achieved. If you could make one for visible photons, you’d make a lot of 
microscopists really happy.

I also had not realized that QE of other detectors is intensity dependent—seems 
counter-intuitive.

JPK

+
Jacob Pearson Keller
Research Scientist / Looger Lab
HHMI Janelia Research Campus
19700 Helix Dr, Ashburn, VA 20147
Desk: (571)209-4000 x3159
Cell: (301)592-7004
+

The content of this email is confidential and intended for the recipient 
specified in message only. It is strictly forbidden to share any part of this 
message with any third party, without a written consent of the sender. If you 
received this message by mistake, please reply to this message and follow with 
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From: Marcus Winter mailto:marcus.win...@rigaku.com>>
Sent: Wednesday, January 16, 2019 11:25 AM
To: Keller, Jacob mailto:kell...@janelia.hhmi.org>>; 
CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] hybrid photon counter in the home lab



Dear Jacob,


Thank you for your reply.  You’re correct, of course.  As shown below and in 
the attachment, with Si sensors and at a photon energy of the 8 keV, the DQE of 
both the HyPix-6000HE and Pilatus3 R 200K HPC detectors are both considerably 
above 90%.


[cid:image001.png@01D4AE4A.E36CAC70]


Most importantly, this high DQE is maintained throughout the operating regime: 
from the highest to the lowest count rates.  As can be seen, this is in 
contrast to the highest performing CCD detector (the Atlas S2) and CMOS type 
detectors.  The high DQE even at the lowest count rates / lowest reflection 
intensities is the reason for the best quality highest resolution data to be 
collectable using the HPC technology detectors.


Many Thanks, Yours sincerely,

Marcus Winter
Rigaku.


From: Keller, Jacob [mailto:kell...@janelia.hhmi.org]
Sent: Wednesday, January 16, 2019 2:13 PM
To: Marcus Winter mailto:marcus.win...@rigaku.com>>; 
CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: RE: [ccp4bb] hybrid photon counter in the home lab

Doesn’t the phrase “each-and-every single photon counting capability” imply 
that quantum efficiency is 100%? I don’t think this is possible—what is the 
quantum efficiency of these detectors?

JPK

+
Jacob Pearson Keller
Research Scientist / Looger Lab
HHMI Janelia Research Campus
19700 Helix Dr, Ashburn, VA 20147
Desk: (571)209-4000 x3159
Cell: (301)592-7004
+

The content of this email is confidential and intended for the recipient 
specified in message only. It is strictly forbidden to share any part of this 
message with any third party, without a written consent of the sender. If you 
received this message by mistake, please reply to this message and follow with 
its deletion, so that we can ensure such a mistake does not occur in the future.

From: CCP4 bulletin board mailto:CCP4BB@JISCMAIL.AC.UK>> 
On Behalf Of Marcus Winter
Sent: Wednesday, January 16, 2019 3:28 AM
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] hybrid photon counter in the home lab



Dear Wolfram,


For the past several years, Rigaku has been installing only HPC (Hybrid Photon 
Counting) X-ray area detector -based diffractometer systems: in conjunction 
with microfocus rotating anode, microfocus sealed-tube and other X-ray sources. 
 (Most recently, we have been deploying the Rigaku HyPix-6000HE HPC detector.)  
The advantages of the HPC technology over any of the phosphor-based detector 
types (CCD, CMOS and CPAD, etc…) are notable: most particularly the discrete 
each-and-every single photon counting capability, extremely high dynamic range, 
small pixel size and the single pixel point function and very rapid read-out.  
With these advantages, significantly higher resolution data can be achieved, - 
and more speedily, compared to the other detector types.  (We have a number of 
established ‘application note’ examples.)  In order to prove the advantages for 
your purposes, the best might be to collect data from a range of your own 
crystal samples.  – There is an open invitation for any seriously interested 
groups to collect data from their crystals in our applications labs.  Please 
contact your local good Rigaku salesperson.

Ma

Re: [ccp4bb] hybrid photon counter in the home lab

[Tim Gruene]

Dear Wolfram,

the principles of data integration do not differ much between small molecule and
macromolecular data (XDS, DIALS, HKL2000, mosflm, and SAINT can be used for
either), hence the advantages reported for chemical crystallography should apply
likewise for macromolecular crystals.

In addition to what is listed for small molecule, the problem of overlapping
reflections due to large unit cell axis might slighlty improve with hybrid pixel
detectors, when the point spread is less than for CCD et al.

Best,
Tim


On Tue, Jan 15, 2019 at 01:20:06PM -0500, wtempel wrote:
> Hi,
> I would value your opinions in this equipment-related question.
> Allé et al have compared detector types with a molybdemon source for a
> small molecule application
> . Are there similar
> published comparisons for protein crystallography? What benefits can I
> expect from replacing a CCD detector with a hybrid photon counter at an
> energy of 8 keV and in the absence of the flux that a modern synchrotron
> provides?
> 
> Thank you in advance.
> Wolfram Tempel
> 
> 
> 
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

-- 
--
Tim Gruene
GPG Key ID = A46BEE1A




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signature.asc
Description: PGP signature

Re: [ccp4bb] hybrid photon counter in the home lab

[Keller, Jacob]

Wow, that’s pretty impressive. I hadn’t realized the high levels of DQE you had 
achieved. If you could make one for visible photons, you’d make a lot of 
microscopists really happy.

I also had not realized that QE of other detectors is intensity dependent—seems 
counter-intuitive.

JPK

+
Jacob Pearson Keller
Research Scientist / Looger Lab
HHMI Janelia Research Campus
19700 Helix Dr, Ashburn, VA 20147
Desk: (571)209-4000 x3159
Cell: (301)592-7004
+

The content of this email is confidential and intended for the recipient 
specified in message only. It is strictly forbidden to share any part of this 
message with any third party, without a written consent of the sender. If you 
received this message by mistake, please reply to this message and follow with 
its deletion, so that we can ensure such a mistake does not occur in the future.

From: Marcus Winter 
Sent: Wednesday, January 16, 2019 11:25 AM
To: Keller, Jacob ; CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] hybrid photon counter in the home lab



Dear Jacob,


Thank you for your reply.  You’re correct, of course.  As shown below and in 
the attachment, with Si sensors and at a photon energy of the 8 keV, the DQE of 
both the HyPix-6000HE and Pilatus3 R 200K HPC detectors are both considerably 
above 90%.


[cid:image001.png@01D4AD9E.60DA3C20]


Most importantly, this high DQE is maintained throughout the operating regime: 
from the highest to the lowest count rates.  As can be seen, this is in 
contrast to the highest performing CCD detector (the Atlas S2) and CMOS type 
detectors.  The high DQE even at the lowest count rates / lowest reflection 
intensities is the reason for the best quality highest resolution data to be 
collectable using the HPC technology detectors.


Many Thanks, Yours sincerely,

Marcus Winter
Rigaku.


From: Keller, Jacob [mailto:kell...@janelia.hhmi.org]
Sent: Wednesday, January 16, 2019 2:13 PM
To: Marcus Winter mailto:marcus.win...@rigaku.com>>; 
CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: RE: [ccp4bb] hybrid photon counter in the home lab

Doesn’t the phrase “each-and-every single photon counting capability” imply 
that quantum efficiency is 100%? I don’t think this is possible—what is the 
quantum efficiency of these detectors?

JPK

+
Jacob Pearson Keller
Research Scientist / Looger Lab
HHMI Janelia Research Campus
19700 Helix Dr, Ashburn, VA 20147
Desk: (571)209-4000 x3159
Cell: (301)592-7004
+

The content of this email is confidential and intended for the recipient 
specified in message only. It is strictly forbidden to share any part of this 
message with any third party, without a written consent of the sender. If you 
received this message by mistake, please reply to this message and follow with 
its deletion, so that we can ensure such a mistake does not occur in the future.

From: CCP4 bulletin board mailto:CCP4BB@JISCMAIL.AC.UK>> 
On Behalf Of Marcus Winter
Sent: Wednesday, January 16, 2019 3:28 AM
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] hybrid photon counter in the home lab



Dear Wolfram,


For the past several years, Rigaku has been installing only HPC (Hybrid Photon 
Counting) X-ray area detector -based diffractometer systems: in conjunction 
with microfocus rotating anode, microfocus sealed-tube and other X-ray sources. 
 (Most recently, we have been deploying the Rigaku HyPix-6000HE HPC detector.)  
The advantages of the HPC technology over any of the phosphor-based detector 
types (CCD, CMOS and CPAD, etc…) are notable: most particularly the discrete 
each-and-every single photon counting capability, extremely high dynamic range, 
small pixel size and the single pixel point function and very rapid read-out.  
With these advantages, significantly higher resolution data can be achieved, - 
and more speedily, compared to the other detector types.  (We have a number of 
established ‘application note’ examples.)  In order to prove the advantages for 
your purposes, the best might be to collect data from a range of your own 
crystal samples.  – There is an open invitation for any seriously interested 
groups to collect data from their crystals in our applications labs.  Please 
contact your local good Rigaku salesperson.

Many Thanks, Yours sincerely,

Marcus Winter
Rigaku.


From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of wtempel
Sent: Tuesday, January 15, 2019 6:20 PM
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] hybrid photon counter in the home lab


Hi,
I would value your opinions in this equipment-related question.
Allé et al have compared detector types with a molybdemon source for a small 
molecule application<https://dx.doi.org/10.1088/0031-8949/91/6/063001>. Are 
there simil

Re: [ccp4bb] hybrid photon counter in the home lab

[Keller, Jacob]

Doesn’t the phrase “each-and-every single photon counting capability” imply 
that quantum efficiency is 100%? I don’t think this is possible—what is the 
quantum efficiency of these detectors?

JPK

+
Jacob Pearson Keller
Research Scientist / Looger Lab
HHMI Janelia Research Campus
19700 Helix Dr, Ashburn, VA 20147
Desk: (571)209-4000 x3159
Cell: (301)592-7004
+

The content of this email is confidential and intended for the recipient 
specified in message only. It is strictly forbidden to share any part of this 
message with any third party, without a written consent of the sender. If you 
received this message by mistake, please reply to this message and follow with 
its deletion, so that we can ensure such a mistake does not occur in the future.

From: CCP4 bulletin board  On Behalf Of Marcus Winter
Sent: Wednesday, January 16, 2019 3:28 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] hybrid photon counter in the home lab



Dear Wolfram,


For the past several years, Rigaku has been installing only HPC (Hybrid Photon 
Counting) X-ray area detector -based diffractometer systems: in conjunction 
with microfocus rotating anode, microfocus sealed-tube and other X-ray sources. 
 (Most recently, we have been deploying the Rigaku HyPix-6000HE HPC detector.)  
The advantages of the HPC technology over any of the phosphor-based detector 
types (CCD, CMOS and CPAD, etc…) are notable: most particularly the discrete 
each-and-every single photon counting capability, extremely high dynamic range, 
small pixel size and the single pixel point function and very rapid read-out.  
With these advantages, significantly higher resolution data can be achieved, - 
and more speedily, compared to the other detector types.  (We have a number of 
established ‘application note’ examples.)  In order to prove the advantages for 
your purposes, the best might be to collect data from a range of your own 
crystal samples.  – There is an open invitation for any seriously interested 
groups to collect data from their crystals in our applications labs.  Please 
contact your local good Rigaku salesperson.

Many Thanks, Yours sincerely,

Marcus Winter
Rigaku.


From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of wtempel
Sent: Tuesday, January 15, 2019 6:20 PM
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] hybrid photon counter in the home lab


Hi,
I would value your opinions in this equipment-related question.
Allé et al have compared detector types with a molybdemon source for a small 
molecule application<https://dx.doi.org/10.1088/0031-8949/91/6/063001>. Are 
there similar published comparisons for protein crystallography? What benefits 
can I expect from replacing a CCD detector with a hybrid photon counter at an 
energy of 8 keV and in the absence of the flux that a modern synchrotron 
provides?

Thank you in advance.
Wolfram Tempel



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1



To unsubscribe from the CCP4BB list, click the following link:
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[ccp4bb] hybrid photon counter in the home lab

[Marcus Winter]

Dear Wolfram,


For the past several years, Rigaku has been installing only HPC (Hybrid Photon 
Counting) X-ray area detector -based diffractometer systems: in conjunction 
with microfocus rotating anode, microfocus sealed-tube and other X-ray sources. 
 (Most recently, we have been deploying the Rigaku HyPix-6000HE HPC detector.)  
The advantages of the HPC technology over any of the phosphor-based detector 
types (CCD, CMOS and CPAD, etc…) are notable: most particularly the discrete 
each-and-every single photon counting capability, extremely high dynamic range, 
small pixel size and the single pixel point function and very rapid read-out.  
With these advantages, significantly higher resolution data can be achieved, - 
and more speedily, compared to the other detector types.  (We have a number of 
established ‘application note’ examples.)  In order to prove the advantages for 
your purposes, the best might be to collect data from a range of your own 
crystal samples.  – There is an open invitation for any seriously interested 
groups to collect data from their crystals in our applications labs.  Please 
contact your local good Rigaku salesperson.

Many Thanks, Yours sincerely,

Marcus Winter
Rigaku.


From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of wtempel
Sent: Tuesday, January 15, 2019 6:20 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] hybrid photon counter in the home lab


Hi,
I would value your opinions in this equipment-related question.
Allé et al have compared detector types with a molybdemon source for a small 
molecule application<https://dx.doi.org/10.1088/0031-8949/91/6/063001>. Are 
there similar published comparisons for protein crystallography? What benefits 
can I expect from replacing a CCD detector with a hybrid photon counter at an 
energy of 8 keV and in the absence of the flux that a modern synchrotron 
provides?

Thank you in advance.
Wolfram Tempel



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1



To unsubscribe from the CCP4BB list, click the following link:
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[ccp4bb] hybrid photon counter in the home lab

[wtempel]

Hi,
I would value your opinions in this equipment-related question.
Allé et al have compared detector types with a molybdemon source for a
small molecule application
. Are there similar
published comparisons for protein crystallography? What benefits can I
expect from replacing a CCD detector with a hybrid photon counter at an
energy of 8 keV and in the absence of the flux that a modern synchrotron
provides?

Thank you in advance.
Wolfram Tempel



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1