[ccp4bb] March 20-21 Workshop in San Diego

Hi Fellows, for the poor frozen souls seeking a defensible excuse for a weekend escape from gloomy winter weather (plus a chance to win a copy of BMC) an educational, tax-deductible opportunity for a nice excursion exists: http://www.ruppweb.org/workshops/Molsoft_2014.htm Modern Drug Target

[ccp4bb] Postdoctoral and senior staff scientist positions in immune and infectious disease

Several postdoctoral and senior staff scientist positions are available in the laboratory of Dr. Zhiwei Huang at Harbin Institute of Technology (HIT, http://en.hit.edu.cn/w2.asp), China. Our research interests focus on the understanding of structure-function relationships of macromolecules, and

[ccp4bb] Postdoctoral position in macromolecular crystallography, HZI Braunschweig, Germany

Postdoctoral Position in Macromolecular Crystallography The Helmholtz Centre for Infection Research (HZI) in Braunschweig (Germany) announces an opening for a postdoctoral researcher in macromolecular crystallography. The position is associated with the department “Structure and Function of

Re: [ccp4bb] Control the crystallization process in the presence of small volatile organic molecules

Hi Chen, I had a similar condition before (reservoir solution with 10% dioxane) and an oil-based cryoprotectant (66.5% w/w paraton-N, 28.5% paraffin oil and 5% glycerol) worked for me. Please check this paper out: http://scripts.iucr.org/cgi-bin/paper?wd5052 In addition, have you tried

[ccp4bb] Examples of multiple ASU copies with different conformations

Hi ccp4bb, I'm putting together a talk for some peers that highlights strengths and weaknesses of structural models for the outsider. For one point, I'd like to find some examples of proteins that show very different conformations between different copies in the ASU. One example I know of is

[ccp4bb] Regisytration open The X6A Workbench - February 25-28,2014

X6A Workbench: Hands-on Synchrotron Structural Biology February 25 - 28, 2014 http://workshops.ps.bnl.gov/default.aspx?w=X6AFeb2014 The NIGMS facility at the National Synchrotron Light Source is offering comprehensive hands-on training in synchrotron data collection and analysis for

[ccp4bb] pdbredo

Dear all I have solved a structure of a protein at 2.1A..the R and free R is 21 and 26..for the betterment of its refinement statistics i submitted the coordinate and structure factor file to we based server PDB_REDO which improved the R and Rfree to 18 and 23, (which probably does through TLS

Re: [ccp4bb] pdbredo

Dear Faisal, The PDB_REDO server returns a .pdb file with residual B-factors after TLS, to submit a model to the PDB it has to have total B-factors. To get those you can run the CCP4 program TLSANL. The program can read the TLS information straight from the .pdb file, but if you have an older

Re: [ccp4bb] Examples of multiple ASU copies with different conformations

The structure of kappa opioid receptor fused with T4 lysozyme (4DJH) contains two copies in the ASU – each copy displays a different orientation between the receptor and lysozyme. On Mon, Jan 27, 2014 at 10:08 AM, Shane Caldwell shane.caldwel...@gmail.com wrote: Hi ccp4bb, I'm putting

Re: [ccp4bb] Examples of multiple ASU copies with different conformations

Hi Shane, One of my favorite examples of large structural differences between molecules in the asymmetric unit have is the crystal structure of the L1 ribozyme (PDB ID 2oiu). The Q and P chains have two stems which crudely co-axially stack and a third stem which appears 180 degrees in the

Re: [ccp4bb] Examples of multiple ASU copies with different conformations

F1 ATPase. Got some Nobel glamour too. Sent from tiny silly touch screen - Reply message - From: Aaron Thompson aaron.a.thomp...@gmail.com Date: Tue, Jan 28, 2014 01:10 Subject: [ccp4bb] Examples of multiple ASU copies with different conformations To: CCP4BB@JISCMAIL.AC.UK The