Re: [ccp4bb] Experimental phasing Selenomethionine data collection etc. tips

Hi Marco, A few suggestions that I like to follow for MAD experiments: Before everything, check you have at least about 1 SeMet per 100 residues Then before crystallisation check by MassSpec that SeMet is properly incorporated in your protein After crystallisation collect first on the peak

Re: [ccp4bb] AlphaFold3 Transparency and Reproducibility

The letter may have had (or helped have) an impact already: On X today from Pushmeet Kohli @ DeepMind "We love the excitement & results from the community on AlphaFold 3 and are doubling the AF Server daily job limit to 20. Happy to also share that we're working on releasing the AF3 model

[ccp4bb] RES: [ccp4bb] What could these crystals be?

It seems to me that your drop has dried. By the pic, I would not say these are crystals Best Rafael Marques da Silva PhD Student – Structural Biology University of Leicester Mestre em Física Biomolecular Universidade de São Paulo Bacharel em Ciências Biológicas Universidade Federal de São

[ccp4bb] Experimental phasing Selenomethionine data collection etc. tips

Hello all, I have a data collection trip next week and plan to collect data on selenomethionine derivative crystals at the al831 beamline. Are there any resources, tips, tutorials, literature etc. That you can recommend to help me prepare for these experiments. Also is there a way to plug in

[ccp4bb] Tenure-track Assistant Professor position - Artificial Intelligence / Machine Learning

I would like to attract your attention to the following position that opens up at The University of Texas Medical Branch (UTMB). Cheers. Matt ​___ Assistant

[ccp4bb] Early Career Diffraction Methods Seminar 2024 - reduced student fees and student grants available!

Dear CCP4BB, A friendly reminder that Early Career Diffraction Methods Seminar 2024 is on the horizon! Taking place July 21-22, 2024 at The Harnack Haus, Berlin, this meeting is designed for early-career scientists to present and explore the latest advancements in diffraction methods, and

Re: [ccp4bb] Fwd: AlphaFold3 Transparency and Reproducibility

If it does what it claims – incredibly impressive. The issue we have is there is no way to verify or validate the claims it is making. The letter is a call and start of a conversation about how the ever-changing landscape of communication and publication of science should be. -Stephanie

Re: [ccp4bb] Fwd: AlphaFold3 Transparency and Reproducibility

It definitely is impressive but it also has clear limitations On 13 May 2024, at 15:22, Sylvia Fanucchi wrote:  You don't often get email from d0c4e77ae410-dmarc-requ...@jiscmail.ac.uk. Learn why this is

Re: [ccp4bb] Request for help in optimizing Coot for AMD 5950x CPU and RX6600XT GPU hardware PC

From: Otsile Mojanaga Sent: Monday, May 13, 2024 3:29 PM To: Paul Emsley Subject: RE: [ccp4bb] Request for help in optimizing Coot for AMD 5950x CPU and RX6600XT GPU hardware PC Dear Paul/All Thank you for the response. I am on Windows 11 and I tried looking around Coot to see where I could

Re: [ccp4bb] What could these crystals be?

Hello, what is in the purification buffer? Best wishes, Jon Cooper. jon.b.coo...@protonmail.com Sent from Proton Mail mobile Original Message On 13 May 2024, 15:15, 张慧欣 wrote: > Hi all > We have been trying with no success to crystalize a protein. Recently we got > these

[ccp4bb] CCP4-BCA Protein Crystallography Summer School (1st – 6th Sept 2024) hosted by the John Innes Centre

Hi All, The John Innes Centre in Norwich (UK) will be hosting this year’s CCP4-BCA Protein Crystallography Summer School. Please follow this link for more details and to apply: https://www.jic.ac.uk/event/ccp4-bca-protein-crystallography-summer-school-2024/ Best wishes Dave

[ccp4bb] Scientific Coordinator post - grade 8 Oxford University

Please forward If you know anyone who might be interested in a drug discovery senior scientific coordinator post ... We are seeking to appoint a Programme Manager/Scientific Coordinator at the University of Oxford's Centre for Medicines Discovery (CMD). The CMD was established in 2020 with the

Re: [ccp4bb] Fwd: AlphaFold3 Transparency and Reproducibility

Is it just me who is really impressed by it? Am I missing something? Get Outlook for Android From: CCP4 bulletin board on behalf of Rafael Marques Sent: Monday, May 13, 2024 3:13:16 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb]

Re: [ccp4bb] Fwd: AlphaFold3 Transparency and Reproducibility

I tried it yesterday and I was really shocked by how fast it is. When I was preparing to submit my second job, the first one was already finished, which made me think that I was definitely doing something wrong. Probably I was...Best wishes Rafael Marques On 13 May 2024 09:53, Harry Powell

Re: [ccp4bb] Request for help in optimizing Coot for AMD 5950x CPU and RX6600XT GPU hardware PC

On 13/05/2024 13:03, Otsile Mojanaga wrote: Dear All I have been using coot as part of my normal workflow but since last week I have been having issues with Coot after I built my own PC which contains the following Hardware: CPU - AMD Ryzen 9 5950X 16-Core Processor - 16 Cores, 32 Threads

[ccp4bb] Request for help in optimizing Coot for AMD 5950x CPU and RX6600XT GPU hardware PC

Dear All I have been using coot as part of my normal workflow but since last week I have been having issues with Coot after I built my own PC which contains the following Hardware: CPU - AMD Ryzen 9 5950X 16-Core Processor - 16 Cores, 32 Threads RAM - 64 GB GPU - AMD Radeon RX 6600 XT -

Re: [ccp4bb] coot - how to define a keyboard shortcut for "update NCS ghosts with local match" ?

On 13/05/2024 11:23, Laurent Maveyraud wrote: Hello, I like the function "update NCS ghosts with local match" found in the calculate/NCT tools menu. It's really helpful when NCS is not really strict. However, I find it clumsy that you have to go in a submenu to get it, so I would like to

Re: [ccp4bb] Postdoctoral position at the Sussex Drug Discovery Centre

Dear ccp4bb community, There are just a few days left to apply for this position - deadline 17th of May. Many thanks We have a postdoctoral position available to join the Drug Discovery team at the Sussex Drug

Re: [ccp4bb] Fwd: AlphaFold3 Transparency and Reproducibility

Hi Harry, Thanks for sharing this. I already read and endorsed the letter. However, I do have a comment - since one of the letter signatories was a reviewer of the paper, wouldn't the shortcomings described in the letter be grounds for rejection of the manuscript? Best regards, Pedro On

[ccp4bb] coot - how to define a keyboard shortcut for "update NCS ghosts with local match" ?

Hello, I like the function "update NCS ghosts with local match" found in the calculate/NCT tools menu. It's really helpful when NCS is not really strict. However, I find it clumsy that you have to go in a submenu to get it, so I would like to define a keyboard shortcut... this would be much

[ccp4bb] In-Silico Structural Biology School - Bari, October 2024

Dear CCP4bb’ers, On behalf of the organizing team and of the Italian Crystallographic Association (AIC) teaching committee, I am pleased to announce this year’s AIC School, organized in partnership with the GENe to Structure (GENS) team (website). Registrations

Re: [ccp4bb] ccp4i2 stopped working (no startup) - advice?

Renato and Stuart McNicholls wrote exactly the right thing: deleting this file resolved the issue. Thank you very much! -Original Message- From: CCP4 bulletin board On Behalf Of Renato Weiße Sent: 08 May 2024 16:44 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] ccp4i2 stopped working

[ccp4bb] Fwd: AlphaFold3 Transparency and Reproducibility

Hi folks This arrived in my inbox this morning, and I believe that it may provoke some discussion… Wikipedia tells me: στέργει γὰρ οὐδεὶς ἄγγελον κακῶν ἐπῶν Best wishes! Harry >From: Stephanie Wankowicz >Sent: Saturday, May 11, 2024 3:31 PM >To: James Fraser ; Pedro Beltrao >

[ccp4bb] Post-doctoral position protein-nucleic acid complexes Granneman lab

I am a group leader at the University of Edinburgh (Granneman Lab), Scotland, UK, and I have a post-doc position available for a structural biologist. The post, funded by a Medical Research Council programme grant, is initially for 2 years but can be