Dear All,
Thanks for the reply.
I will try process and refine the data again, will see if it improves.
Thanks again to all.
--
Sonali Dhindwal
“Live as if you were to die tomorrow. Learn as if you were to live forever.”
From: Mark van Raaij
There isnt much information here!
Funny that 3 chains are poor - does that mean one and a half heterodimers?
I presume you have checked spacegroup? Zanuda will test to see if any
higher symmetry is present..
Eleanor
On 29 April 2013 06:02, sonali dhindwal sonali11dhind...@yahoo.co.inwrote:
Dear Eleanor,
Thanks for the suggestion, I just checked on the Zanuda program, it is also
giving P1 as the best possible spacegroup for the molecule.
and by not refining well, I meant for the electron density which is broken at
many places at main chain, and poor electron density for the
FYI, I do know of one example of a solved structure where some of the
molecules in the ASU are poorly defined. In 1EKJ, 4 of the 8 molecules
in the ASU have low B-factors (mid 30s) and 4 have high B-factors
(50s-60s). In the unit cell these layers alternate. It is possible, if
everything else
You may also get some insights from TLS refinement.
Regards
Thierry
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Roger
Rowlett
Sent: Monday, April 29, 2013 12:27 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Poor electron density in some of the chains in an
Dear All,
Thanks for the help and suggestion
Francis, we used molrep for the structure solution. As I told that we already
have the structure of the same protein, but a variant. So, we used its single
chain (heteromer) as a model for molecular replacement.
and Eleanor, I deleted once that
On 04/29/13 12:26, Roger Rowlett wrote:
FYI, I do know of one example of a solved structure where some of the
molecules in the ASU are poorly defined. In 1EKJ, 4 of the 8 molecules
in the ASU have low B-factors (mid 30s) and 4 have high B-factors
(50s-60s). In the unit cell these layers
and 2VAK...average Bs for the twelve, sequence identical, chains vary from 28
to 53.
On 29 Apr 2013, at 22:09, David Schuller wrote:
On 04/29/13 12:26, Roger Rowlett wrote:
FYI, I do know of one example of a solved structure where some of the
molecules in the ASU are poorly defined. In
Dear All,
We are working on a crystal structure with 12 molecules in an asymmetric unit.
It is a heteromer and each chain is made up of two type of chains. Therefore
there are 24 chains in an asymmetric unit.
It is giving solution in P1. Rfactor and Rfree has reached 20.25 and 25.6 at a
