Dear all,
I will give my advice once again and hopefully you will discuss on it the
next 2 days :P
Chris might be worth trying a 2ndary or 3ary structure prediction tool to
ensure that you do not have f.i. flexible ends.
Disordered regions may lead to this kind of issues, as well.
Personally I
Hi,
I was in Sung-Hou Kim's group when this work below was performed and
published and I also tried it out on many occasions. Elegant piece of
work and certainly worth trying.
Aside from the suggestions of trying different pH and related optimum
solubility screening and if higher salt and
I'd try varying the pH independent of the theoretical pI, sometimes the real pI
is very different.
(I've worked on a protein with theoretical pI 9.2, real pI determined by
iso-electric focussing 7.8).
I'd also try limited proteolysis on the milky sample and see if you can
solubilise it while a
On behalf of the organising committee I would like to publicise the upcoming
third edition of the Congress "ICG - Italian Crystal Growth", which will be
held the next Fall on November 20-21, 2017 at Università degli Studi di
Milano-Bicocca in Milan (Italy).
For some years now, this Conference
hi folks
Just my two ha'porth - the small molecule crystallographers have been doing
multi-orientation data collections since they moved from point detectors to
area detectors in the early 1990's, for the very reasons that Gerard gives
(their cusps are huge compared to ours...). Since they
Just to comment on what Graeme just introduced. We (and I know we are not the
first ones and not the only ones) are pushing our user community towards this
procedure as a standard: lowering the transmission (less juicy, yet...) and
getting few data with various chi. It does help greatly in
Attenuation... cut the beam with primary slits! We do not use attenuators, only
for getting very very low when performing energy scans. Else, cutting the flux
at the source is somehow much more reliable. Not mentioning scattering coming
from the attenuators as well...
Cheers, leo
-
Leonard
Reading back my email, when I mentioned 'just introduced', it is not giving
justice to the reality and those who came up with the concept. I should have
mentioned 'just reminded us', as the concept has been introduced quite a long
time ago and few tens of communications. It is therefore a
I'm not understanding much of this discussion, but I've noticed the use of
words reminiscent of old issues concerning omega-two theta scans on four-circle
goniostats (see Stout and Jensen, pages 168-173, second edition).
p.s. In my upbringing, crystals were placed on goniometer heads so they
Hi Chris,
Does you protein polymerize?
We had examples that protein solution turned turbid at high temperature and
become aggregates. We also had proteins precipitated on ice but not at 4C.
Sometimes not much protein loss after clearance.
Looks like your Ni-NTA buffer (500 mM NaCl, 30 mM
And the instrument makers (e.g., Rigaku and Bruker) currently sell multi-axis
goniostats for in-house data collection--and not just for small-molecule
purposes.
Diana
P.S. Ron, we still call 'em goniometer heads and goniostats at UT Southwestern.
> On Jul 14, 2017, at 1:00 PM,
I did not follow all the responses to this, but, did you try to use the Ni-NTA
buffer (500 mM NaCl, 30 mM HEPES pH 7.5, 10% glycerol) to run the gel
filtration?
Yuzhu.
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Chris Fage
Sent: Friday, July 14, 2017 3:33 AM
To:
The Phenix developers are pleased to announce that version 1.12 of Phenix is
now available (build 1.12-2829). Binary installers for Linux, Mac OSX, and
Windows platforms are available at the download site:
http://phenix-online.org/download/
Highlights for this version:
New tools for
Hi,
We have recently noticed an issue with our Pilatus (biased pixels/vertical
lines).
I was curious as to whether anyone else has seen this or might know what could
have caused it?
Best,
John
Dear all,
We have two open postdoc fellowships in my group at Umeå University,
Sweden. Each of the projects could be suitable for a person with
background in X-ray crystallography and biochemistry eager to expand
their methods scope to single particle cryo-EM and/or membrane
reconstitution
Dear Leo,
What seems to have happened is that an existing thread where fine
phi (actually: omega!) slicing was discussed, among many other things,
digressed into a discussion of data collection protocols using more
than one instrumental setting (either using a 2-theta motion of the
detector,
Dear All,
Thank you for the many suggestions. After sending my first message to the
BB, I tried exchanging the sample into buffer containing 5 mM EDTA and also
into buffer at pH 9.0 (using BICINE). Neither of these appear to have
helped the instability--precipitation still occurred within ~1 min
Hi Graeme,
I see your point about the blind region and also the tile lines. But 2-theta
would have the advantage of also shifting the low-res spots to entirely new
pixels, which would be harder through rotation. Also, wouldn't rotating about
the beam axis shift the spots to variable degrees
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